摘要
目的 :观察人重组碱性成纤维细胞生长因子 (hu manbasicfibroblastgrowthfactor,hbFGF)对人牙周膜细胞(periodontalligamentcells,PDLC)增殖和分化的效应 .方法 :对hbFGF (5 0 μg·L-1 )刺激后 3,9,1 5和 2 1d的细胞进行计数 ,同时行HE和免疫组织化学染色 .结果 :hbFGF (5 0 μg·L-1 )可促进PDLC细胞生长 ,在培养的 3,9,1 5和 2 1d时 ,其细胞计数分别是 38× 1 0 3 ,80× 1 0 3 ,1 0 6× 1 0 3 ,99× 1 0 3 .但不改变PDLC的形态和生长方式 ;与对照组相比PDLC的ConA ,WGA凝集素受体表达无差异 .两组细胞均未出现钙化现象 .结论 :hbFGF可促进体外PDLC的增殖 。
AIM: To evaluate the effects of human basic fibroblast growth factor (hbFGF, 50 μg·L -1 ) on the proliferation and differentiation of human periodontal ligament cells (PDLC) in vitro . METHODS: The number of cells was counted at different time (3, 9, 15 and 21 d) after adding hbFGF into the culture medium and HE and immunohistochemistry staining were conducted. RESULTS: hbFGF (50 μg·L -1 ) stimulated the cells proliferation and didn't change the morphology and growth mode of the cells. There was no significant difference in expression of ConA and WGA receptors between the two groups and no mineralized extracellar matrix of cells was observed. CONCLUSION: hbFGF may stimulate the proliferation of human periodontal ligament cells in vitro, but no effects on the phenotype of cells have been obseved in this experiment.
出处
《第四军医大学学报》
北大核心
2003年第23期2126-2128,共3页
Journal of the Fourth Military Medical University
关键词
碱性成纤维细胞生长因子
牙周膜细胞
凝集素受体
体外
basic fibroblast growth factor
periodontal ligament cells
agglutinin receptor
in vitro