ET-1与梗阻性黄疸冷应激肾损伤

Effects of plasma endothelin-1 on renal damage in obstructive jaundiced rats under cold stress condition

目的 :了解血浆内皮素 1 (ET 1 )的水平及与肾功能损害的关系 .方法 :采用放免法测定血浆ET 1含量 .结果 :胆总管结扎 (BDL)应激ET 1 (2 0 6 .72± 34.31 )ng·L-1 明显高于BDL对照组 (1 6 8.6 3± 2 6 .5 8)ng·L-1 ,(P <0 .0 5 ) .BUN(1 2 .31± 3.1 4 )mmol·L-1 和Cr (71 .6 4± 9.4 1 )mol·L-1也较BDL对照组升高 (P <0 .0 1 ) .BDL加ET抗血清应激组ET 1水平 (1 6 8.75± 32 .4 )ng·L-1 较BDL应激组降低 (P <0 .0 5 ) ,BUN和Cr均也下降 ,ET 1与BUN ,Cr均呈正相关(rS=0 .74 3,rS=0 .831 ) .结论 :梗阻性黄疸大鼠血浆ET 1在应激状态下明显升高 .与BUN和Cr呈正相关 .

AIM: To study plasma endothelin 1 levels of rats with obstructive jaundice under cold stress and to investigate its relation to renal damage. METHODS: The rat models of biliary duct ligation ( BDL) were established and plasma endothelin 1 (ET 1) was measured by radioimmunassay. RESULTS: Plasma ET 1 levels of BDL stress group (206.72±34.31) ng·L -1 was significantly higher than those of BDL control (168.63±26.58) ng·L -1 ( P < 0.05) . Serum BUN and Cr of BDL stress group increased significantly compared with that of BDL control group ( P < 0.01) . Plasma ET 1 level of BDL Ab stress group (168.75± 32.4) ng·L -1 decreased ( P <0.05), and BUN and Cr were lower than those of BDL stress ( P <0.05). There was a positive correlation between ET 1 levels and BUN and Cr ( r S=0.743, r S=0.831). CONCLUSION: Plasma ET 1 levels increase under the cold stress condition and worsen the renal damage of obstructive jaundiced rats.