酵母双杂交系统测定抗雌激素物质

Determination of Chemical Compounds Using Antagonist AssayBased on Yeast Two-Hybrid System

建立了用酵母双杂交系统筛选具有抗雌激素作用物质的生物测试法 ,并对 6种工业用酚类物质 ( 4 n 壬基酚 ,2 sec 丁基酚 ,2 羟基联苯 ,3 叔丁基酚 ,2 叔丁基酚和 4 乙基 2 ,6 二叔丁基酚 )及氯化三苯基锡的抗雌激素作用进行了测试。结果表明 :氯化三苯基锡显示出极强的抗雌激素作用 ,强度超过了抗乳癌剂三苯氧胺及其代谢产物 4 羟基三苯氧胺。可以推测出有机锡造成软体动物的雄性化作用可能是由其抗雌激素作用引起的。 6种酚类物质中 ,除 4 乙基 2 ,6 二叔丁基酚外 ,其他 5种显示出弱抗雌激素作用。值得注意的是 :当 4 壬基酚显示出雌激素促进作用时 ,4 n 壬基酚却显示出抗雌激素作用。

The estrogenic antagonist activities of triphenyltin chloride and 6 phenolic compounds (4-n-nonylphenol,2-sec-butylphenol,2-hydroxybiphenol,3-tert-butylphenol,2-tert-butylphenol and 4-methyl-2,6-di-tert-butylphenol) used in the coating of concrete tanks and lining of still pipe in water supply system were detected using antagonist assay based on yeast two-hybrid system.It was found that triphenyltin chloride elicits strong antagonist activity of which is even higher than those of tamoxifen and its metabolized product,4-hydroxytamoxifen.Of six phenolic compounds,five objective phenolic compounds elicited weaker estrogen antagonist activity except for 4-methyl-2,6-di-tert-butylphenol.It should be noted that 4-n-nonylphenol elicited antagonist activity,while the 4-nonylphenol elicited agonist activity.