超高产杂交水稻皮秒分辨荧光特性(英文)

Properties of Picosecond Fluorescence of Super High-Yield Hybrid Rice

采用时间分辨荧光光谱测试系统,研究了超高产杂交水稻(Oryza sativa L.)两优培九(P9)和对照汕优63(SH 63)类囊体膜的荧光光谱特性和时间特性。以脉宽为120 ps,重复率为4 MHz,波长为514 nm的Ar+激光分别激发P9和SH 63水稻类囊体膜荧光。通过对其超快荧光的时间特性和光谱特性比较研究发现:无论是在灌浆期还是在扬花期,P9水稻类囊体膜中光系统Ⅰ激发能传递的速度比光系统Ⅱ 的快;P9和SH 63两种水稻类囊体膜在灌浆期的激发能传递速度都比扬花期的快;两种水稻类囊体膜的光谱特性还给出,在从扬花期到灌浆期这一生长发育过程中,SH 63水稻类囊体膜的色素成分和结合状态发生了变化,而P9却没有出现这种变化。

Thylakoid membrane preparations of super high-yield hybrid rice (Oryza sativa L.), Liangyoupeijiu (P9) and Shanyou 63 (SH 63) were used for investigating its spectral and time properties by using picosecond time-resolved fluorescence spectrum measuring system. The thylakoid membrane preparations of P9 and SH 63 were excited by an Ar+ laser with a pulse width of 120 ps, repetition rate of 4 MHz and wavelength of 514 nm. The time constants of the excited energy transfer in these two varieties at flowering stage and grain filling stage were calculated from the experimental data. Based on the comparative studies of the time and spectral properties of the excited fluorescence in these ultrafast dynamic experiments the following was found: at both the flowering stage and grain filling stage, the speed of the excitation energy transfer, in photosystem was faster than that in photosystem II in P9 variety; and the speed of the excitation energy transfer at grain filling stage was faster than those at flowering stage for both rice varieties; the experiments also implied that the components and assembly of pigments in SH 63, but not in P9, changed during the process from flowering stage to grain filling stage for in these two rice varieties.