参杞合剂对小鼠腹水型肝癌细胞株H22细胞周期及凋亡的影响

Effects of Shenqi compound recipe on cell cycle and apoptosis of ascites inducible hepatocarcinoma cell line H22

目的 :观察参杞合剂 (SQ)在体内、外对小鼠腹水型肝癌细胞株 (HcaF16A3 )细胞周期及凋亡的影响。方法 :用SQ对荷瘤小鼠连续胃饲治疗 10d ,观察其NK细胞 /Mφ的杀伤活性及细胞周期的改变。用流式细胞仪检测SQ对HcaF16A3 细胞增殖的抑制作用与诱导凋亡的作用。结果 :SQ的抑瘤率为6 5 .6 8%。流式细胞仪检测发现 ,SQ可使肿瘤细胞的细胞周期阻滞到S期 ,并在体外诱导其凋亡。结论 :SQ在体内、外均有抑瘤作用 。

AIM: To observe the effects of Shenqi compound recipe(SQ)on cell cycle and apoptosis of hepatocarcinoma cell line H22 in vitro and in vivo . METHODS: BALB/c mice bearing tumors were perfused with SQ for 10 days runming. And then cytotoxic activity of mouse NK cells and macrophages and variation of cell cycle of H22 cells were observed. Inhibition of H22 cell proliferation and induction of the their apoptosis by SQ were analyzed by flow cytometry. RESULTS : The tumor suppre ssive rate of SQ was 65.68% . SQ could make H22 cells arrested at the S phase and induce their apoptosis. CONCLUSION: SQ has significant anti tumor effect both in vitro and in vivo . Its mechanism may be related to blocking of cell cycle and inducing apoptosis of H22 cells.