结合脂蛋白_(136-150)修饰抗原在体内外对4B.14a T细胞克隆的作用

Effects of altered peptide ligands (APLs) of proteolipid_(136-150) on T-cell clone 4B.14a

目的 :通过研究结合脂蛋白13 6 150 (PLP13 6 150 )及其修饰抗原在体内外对T细胞克隆 4B .14a的影响 ,进一步探讨修饰抗原防治多发性硬化 (MS)的可行性。方法 :在体内 ,为模拟MS复发的临床过程 ,首先将SJL/J小鼠用X线辐射 4 5 0R ,静脉转移无分裂增殖能力的 4B .14a细胞 (1× 10 7/鼠 ) ,再用 5 0 μg/鼠PLP13 6 150 修饰抗原免疫动物 ,以触发被动实验性变态反应性脑脊髓炎 (EAE )。在体外观察PLP13 6 150 及其修饰抗原刺激4B .14a细胞的增殖作用和分泌细胞因子的作用。结果 :除139A、14 3A、14 4A、14 5A和 14 8A外 ,其他修饰抗原均可在体内触发 4B .14aT细胞引起被动EAE。对PLP13 6 150 和其大多数修饰抗原 ,4B .14a细胞表现为增殖反应 ,分泌炎性细胞因子 ,对 14 3A、14 4A和 14 8A刺激的反应较弱 ;139A可抑制4B .14a细胞增殖。结论 :PLP13 6 150 的某些修饰抗原在体内外对 4B .14aT细胞克隆具有不同的作用 。

AIM: By investigating the effects of APLs of PLP 136 150 on T cell clone 4B.14a in vitro and in vivo , to determine the feasibility of APLs to prevent relapsing multiple sclerosis (MS) in human. METHODS: To mimic the clinic course of relapsing MS, SJL/J female mice were first irradiated at 450R and intravenously infused with 1×10 7 resting 4B.14a T cells per mouse. Then mice were immunized with 50 μg/mouse APL for inducing passive experiment allergic encephalomyelitis (EAE) . The proliferation and cytokine production of 4B.14a T cells in response to APLs were also examined. RESULTS: Except 139A, 143A, 144A, 145A and 148A, other APLs triggered T cells to induce passive EAE; 4B.14a T cells well responded to the most APLs, weakly responded to 137A, 144A and 148A, but the response was suppressed by 139A. CONCLUSION: The effects of some APLs on 4B.14a T cells are different in vitro and in vivo . It may be feasible to select some APLs to prevent relapsing MS in human.