中国鲎鲎抗脂多糖因子的提取、纯化及其活性的初步鉴定

Extraction, Purification and Preliminary Activity Assay of Limulus Anti-lipopolysaccharide Factor from Chinese Horseshoe Crabs

目的 从中国鲎血细胞中提取鲎抗脂多糖因子并初步鉴定其活性。 方法 采用超滤、浓缩、离子交换层析等方法提取、纯化中国鲎鲎抗脂多糖因子 ,应用鲎变形细胞溶解物抑制试验测定其活性。 结果 初步纯化得到的鲎抗脂多糖因子经 SDS- PAGE鉴定其纯度达 90 %以上 ,分子量为 1 5 .6 8k D,在体外可中和脂多糖 (L PS)凝固鲎溶解物的活性 ,其中和比例约为 5 0 0∶ 1。 结论 初步纯化的中国鲎鲎抗脂多糖因子具有中和 L

Objective\ To isolate and purify limulus anti\|lipopolysaccharide factor from Chinese horseshoe crabs and test its bioactivity in lipopolysaccharide(LPS) neutralization.\ Methods\ Limulus anti\|lipopolysaccharide factor from Chinese horseshoe crabs was purified by ultrafiltration and ion\|exchange chromatography.\ Its activity was detected by limulus amoebocyte lysate inhibited test.\ Results\ The purity of purified limulus anti\|lipopolysaccharide factor from Chinese horseshoe crabs reached more than 90% by SDS\|PAGE.\ Its molecular weight was 15 68 kD.\ It could inhibit LPS conglutination in vitro.\ The ratio of limulus anti\|lipopolysaccharide factor from Chinese horseshoe crabs for complete neutralization to LPS was 500∶1.\ Conclusion\ The purified limulus anti\|lipopolysaccharide factor from Chinese horseshoe crab could inhibit activity of LPS in vitro.\;