哮喘豚鼠内皮素-1及其基因表达和雷公藤内酯醇的影响

Effects of triptolide on endothelin-1 and its gene expression in lung of asthmatic guinea-pigs

目的 研究哮喘豚鼠肺组织中内皮素系统基因表达和雷公藤内酯醇(TP)的影响。方法 放免法测定肺组织ET-1,逆转录多聚酶链式反应(RT-PCR)方法检测ET-1、ETA、ETB和ECE的mRNA表达量。结果 ①哮喘组各指标均有不同程度的升高。首次激发哮喘后,ET-1在4 h升高最明显,而ET-1mRNA、ETAmRNA和ECEmRNA均在2 h升高最明显,较对照组增加(P<0.01),ETBmRNA升高不明显。哮喘2 wk组除ECEmRNA外,各指标均增加(P<0.01,P<0.05)。②较哮喘组,TP干预组在首次激发哮喘后,各指标即刻均有下降,但差异不显著,2～8 h后下降明显(P<O.01,P<O.05)。干预2 wk组除ECEmRNA外各指标均低于哮喘2 wk组(P<0.01,P<O.05)。结论 哮喘时肺组织ECE、ET-1及其受体基因表达有不同程度的升高,TP抑制ET-1系统基因的表达。

AIM To explore the effects of trip-tolide on endothelin-1 and its gene expression in lung of asthmatic guinea-pigs. METHODS The contents of ET-1 in lung of asthmatic guinea-pigs were measured with radioimmunoassay, and the expressions of ET-1, ETA, ETB and ECEmRNA with reverse transcription DNA polymerase chain reaction (RT-PCR). RESULTS 0 All of the indexes were increased after guinea-pigs were induced asthma. At the 4th hour after first induction of asthma, ET-1 reached the top, but at the 2rd hour, ET-1mRNA, ETAmRNA and ECEmRNA were markedly higher than control group (P <C 0. 01). In two-week asthma group, all of indexes but ECEmRNA increased markly ( P < 0. 01 -0. 05). (2) TP can decreased all the indexes after 2 -8 hours' asthma (P< 0. 01 - 0. 05), and two weeks later all of the indexes were significantly lower than those in asthma groups. CONCLUSION There was markedly higher ET-1,ETA, ETB and ECE mRNA expression in the lung in asthmatics, and TP can inhibit the expression of these genes.