猕猴胚胎干细胞的诱导分化和凋亡

Induced Differentiation and Apoptosis of Rhesus Monkey Embryonic Stem Cells

采用单层培养法研究维生素A酸 (RA)、神经生长因子 (NGF)、上皮生长因子 (EGF)和碱性成纤维生长因子 (bFGF)对猕猴胚胎干细胞系R36 6 4的诱导分化和凋亡的作用。结果表明 :①不添加任何生长因子的条件下 ,细胞分化不定向 ,各种细胞所占的比例表现出明显的随机性 ;②添加单一生长因子能促进细胞的分化进程 ,并使某一类或某几类的分化细胞比例上升 ,RA和NGF均能促进神经样细胞的形成 ,EGF促进内皮样细胞的形成 ,bFGF提高成纤维样细胞的比例 ;③在分化的过程中伴有细胞早期和晚期凋亡的发生 ,RA和NGF可增加细胞凋亡的数量。这种由生长因子诱导的动物胚胎干细胞的分化可能存在种间差异。

To investigate the effect of retinoic acid (RA),nerve growth factor (NGF),epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on the differentiation and apoptosis of rhesus monkey embryonic stem (rES) cell line R366.4,rES cells were cultured in monolayer state in vitro,and the experiment resulted in:①The percentage of differentiated rES cells increased along with the elongated duration of culture.②The rES cells differentiated into heterogeneous somatic cells,whose proportions were at random,in the absence of exogenous growth factors.③Single growth factor could promote progress of differentiation,and increased the proportion of one or several types of differentiated cells;neuron-like cells increased in the presence of RA or NGF,while endothelium-like cells and fibroblast-like cells increased in the presence of EGF and bFGF,respectively.④During the differentiation of rES cells the early and the late apoptosis happened,and the apoptosis could be promoted by RA and NGF.The four growth factors have the potential to stimulate differentiation of rES cells in different ways.The ES cell differentiation induced by the growth factor may be species-specific.