幽门螺杆菌细胞微生物学模型的研究

Establishment of a model to study Helicobacter pylori cellular microbiology

目的 建立幽门螺杆菌 (Helicobacterpylori,Hp)细胞微生物学研究模型。方法 选择 6至 8月龄胎儿胃粘膜组织进行原代细胞培养 ,运用扫描和透射电镜研究了螺杆状和圆球形Hp与人胃粘膜上皮细胞相互作用关系。结果 螺杆状Hp很快与人胃粘膜上皮细胞微绒毛粘附 ,Hp粘附细胞处形成一个致密纤维样肌动蛋白垫 ,上皮细胞膜杯状内陷包绕Hp ,紧密粘附于宿主细胞 ,Hp粘附下方细胞内微丝、微管和肌动蛋白呈局灶性聚集。圆球形Hp可出现类似生物学效应 ,提示两种形态的Hp皆可作用于人胃粘膜上皮细胞。此外 ,螺杆状Hp还可与宿主细胞膜融合 ,被细胞内在化 ,细胞内出现空泡效应。上述病理性改变与Hp感染的胃粘膜活性组织标本的检查结果基本一致。结论 原代胃粘膜上皮细胞与Hp相互作用近似于Hp体内致病的微环境 ,是Hp细胞微生物学研究的良好模型。

Objective To establish a useful model to study H.pylori cellular microbiology. Method We cultured human gastric epithelial cells, which were taken from aborted fetus (6-8 months old). Characteristics of the spiral and coccoid form of H.pylori attachment to human gastric cell were examined by scanning and transmission electron microscopy. Results Spiral H.pylori soron adhered to microvilli. Specialized attachment sites such as the 'adhesion pedestal', 'cup-like indentation', 'abutting adhesion' were seen. H.pylori stimulated microfilament, microtubule and actin polymerization, which was associated with adherence pedestal formation. These adherence patterns were similar to those observed in vitro, suggesting a possible pathogenic role for the coccoid of H.pylori . We observed, that the spiral H.pylori, fused with the epithelial cell membrane. Some bacteria were internalized by the host cell. Meanwhile attachment of spiral H.pylori resulted in effacement of microvilli and vacuolation effect on gastric epithelial cells. Conclusion Primary gastric cell cultures closely imitate the entero-environment and is a potential useful model to study H.pylori. [