电针对面神经损伤后再生室中神经生长因子的影响

Effect of Electroacupuncture on NGF Level in the Injured-facial Nerve Regeneration Chamber

目的 :了解电针对面神经损伤后再生微环境中神经营养因子 (NGF)的影响。方法 :采用同龄成年健康的新西兰兔 50只 ,随机分为针刺组和对照组 ,均在 3 %的戊巴比妥钠静脉麻醉下 ,分离暴露面神经上颊支 ,在手术放大镜下切断神经 ,用硅胶管将两断端嵌入并缝合固定 ,形成再生室。针刺组于术后当天完全醒后开始接受电针治疗 ,每天 1次 ,对照组不作任何处理。术后3天、5天、7天、1 0天、1 4天分别处死 1 0只动物 ,针刺组与对照组各 5只。抽取再生室内液体 ,两组对照。结果 :在伤后 3～ 7天 ,针刺组和对照比较再生室内NGF浓度差别无显著性 (P >0 .0 5) ,接受电针治疗 1 0天和 1 4天 ,针刺组再生室内NGF浓度比对照组高 (P <0 .0 1 ) ,伤后第 7天 ,针刺组和对照组再生室内NGF浓度均达到峰值 ,但对照组在伤后 2周开始降低 ,针刺组仍保持在较高的水平。结论 :电针对面神经损伤后再生微环境中神经生长因子 (NGF)的浓度有提高其水平和维持平稳水平的作用 。

Objective: To observe the effect of electroacupuncture (EA) on NGF level in the injured facial nerve regeneration chamber in the rabbits. Methods: Facial nerve injury was induced by transectioning the upper buccal branch of the facial nerve about 5 mm in length that was then fixed in a 12 mm long silica gel tube (chamber). 50 facial nerve injury rabbits were randomized into control and EA groups, with 25 cases in each group. EA (18～20 Hz, 1.5 V, 30 min/time) was applied to 'Yifeng' (TE 17), 'Dicang' (ST 4) to 'Jiache' (ST 6) and 'Hegu' (LI 4), once daily, continuously for 3～14 days. EA and control groups were respectively divided into 3 d, 5 d, 7 d, 10 d, 14 d subgroups. The fluids samples accumulated in the chamber were collected at different postoperative intervals: 3 d, 5 d, 7 d, 10 d, 14 d for detecting NGF content using double antibody Sandwiched ELISA. Results: In 7 d control and EA subgroups, NGF levels peaked, and decreased in 10 d and 14 d control subgroups, suggesting that EA can maintain the higher secretion level. NGF contents of the 5 EA subgroups particularly 10 d and 14 d subgroups ( P <0.01) were all higher or significantly higher than those of the corresponding 5 control subgroups, indicating that EA treatment can potentiate the secretion of NGF of the injured facial nerve and the effect of EA peaked on the 10 th day. In 7 d control and EA subgroups, NGF levels peaked, and decreased in 10 d and 14 d control subgroups, suggesting that EA can maintain the higher secretion level. Conclusion: Electroacupuncture therapy can promote the secretion of NGF of the injured nerve which may contribute to the effect of acupuncture in promoting the regeneration of the injured nerve.