穴注黄芪、当归注射液对实验性慢性萎缩性胃炎胃粘膜血流的影响

Effects of Acupoint-injection of Huangqi and Danggui Injectio on Gastric Mucosal Blood Flow in Chronic Atrophic Gastritis Rats

目的 :探讨穴注黄芪、当归注射液对慢性萎缩性胃炎 (CAG)胃粘膜血流的影响。方法 :采用N 甲基 N 硝基亚硝基胍 (MNNG)诱发大鼠CAG。随机将 70只大鼠分为正常组、模型 1组( 40 μg/mLMNNG造模 )、模型 2组 ( 60 μg/mLMNNG造模 )、穴注 1组 ( 40 μg/mLMNNG造模+穴注 )与穴注 2组 ( 60 μg/mLMNNG造模 +穴注 )。穴注组均于造模第 1 0周开始以黄芪、当归注射液等份混合注入“足三里”穴。造模第 3 1周时观察各组大鼠胃粘膜病理及胃粘膜血流的改变。结果 :随造模浓度的增加 ,大鼠胃粘膜血流量下降 ,胃粘膜损伤加重 ,二者呈负相关 ;同时胃粘膜肠化及异增程度加重 (P均 <0 0 5)。穴注组可明显增加胃粘膜血流量 ,减轻胃粘膜损伤 (P <0 0 5)。结论 :局部血流量减少是胃粘膜损伤与萎缩发生、进展至胃癌前病变的重要原因 ;穴注法可能通过改善胃粘膜血供、促进组织修复而防治CAG。

Objective: To investigate the effects of acupoint injection of Danggui (Radix Angelicae Sinensis)injectio and Huangqi (Radix Astragali seu Hedysari) injectio on blood flow of the gastric mucosa in chronic atrophic gastritis (CAG) rats. Methods:SD rat CAG model was made by feeding the rats with different concentrations of N methyl N' nitro N nitrosoguanidine (MNNG) continuously for 10 weeks. 60 CAG rats were randomly assigned to model group Ⅰ (40 μg/ mL MNNG, n =15) and model group Ⅱ ( 60 μg/mL MNNG, n =15),acupoint injection group Ⅰ ( 40 μg/mL MNNG, n =15 ) and acupoint injection group Ⅱ ( 60 μg/mL MNNG, n =15). In addition, other 10 normal rats were used as control group. In these 2 acupoint injection groups,the mixture solution of Huangqi and Danggui injection (0.1 mL) was injected into unilateral 'Zusanli' (ST 36), once every other day, continuously for 20 weeks. Pathological changes of gastric mucosal tissues (the tissue sections were stained with HE method) were examined and gastric mucosal blood flow was then determined. Results: Along with the increase of the concentration of the administered MNNG, the degrees of gastric mucosal injury (shallow and deep erosion, ulcer)were increased significantly( P < 0.05 )and the gastric mucosal blood flow in the anterior region,the body part and the antral region of the stomach decreased remarkably in comparison with normal control group( P < 0.01), which presented a negative correlation between the mucosal injury and the blood flow( P < 0.05); pathologic changes of mucosal injury index, atrophy, intestinal metaplasia and dysplasia of the gastric mucosa increased significantly( P < 0.05). In acupoint injection groups,the degrees of the above mentioned gastric mucosal injury were remarkably improved( P < 0.01) and the gastric mucosal blood flow volume was significantly increased in comparison with the corresponding same MNNG concentration model groups( P < 0.05). Conclusion: The decrease of gastric mucosal blood flow plays a key role in pathogenesis of gastric mucosal injury, atrophic and gastric precancerous changes. Acupoint injection therapy may prevent and treat CAG by improving gastric mucosal blood flow supply and promoting repair of the injured gastric tissue.