脑干损伤致死星形胶质细胞GFAP和ET-1的表达

Study on GFAP changes of astrocyte and expression of endothelin-1 in brainstem injury of human being

目的 研究星形胶质细胞GFAP和ET-1的表达在脑干损伤的分子病理学改变并探讨对脑干损伤法医学鉴定中的意义。方法 筛选本中心提供的不同时间急性脑干损伤死亡的脑标本15例及非颅脑损伤死亡标本5例,HE染色常规观察各部位脑组织的显微形态改变之后,用检测单克隆抗体免疫组化SP方法检测胶质细胞GFAP和ET-1的表达,研究其病理变化。结果 生前急性脑干损伤部位GFAP阳性细胞数目增多,细胞形态变大,存在ET-1的表达。结论 上述系列病理变化对于法医实践中急性脑干损伤的死后诊断具有参考价值。

Objective To study GFAP changes of astrocyte and expression of Endothelin-1 in molecular pathology in brainstem injury of human being and explore its forensic medical significance. Method 15 brain specimens of acute brainstem injury and 5 brain specimens of no brainstem injury in different time were selected and their microscopic morphological changes were observed with HE staining, and we study the pathological changes of GFAP of astrocyte and expression of Endothelin-1 in monoclonal antibody im-munohistochemistry SP method. Results The number of GFAP positive cells was increased, the bulk of the cell became bigger and expression of Endothelin-1 could be observed. Conclusion The above pathological changes might be a valuable base used for postmortem diagnosis of acute brainstem injury.