兔骨髓间充质干细胞定向诱导分化为脂肪细胞及成骨细胞的研究

Study on Rabbit Mesenchymal Stem Cells Differentiation to the Adipogenic or Osteogenic Lineage in vitro

用密度梯度离心和贴壁法分离和纯化兔骨髓间充干细胞 ,建立诱导兔 MSCs向脂肪细胞及成骨细胞表型转化的方法及条件。在成脂诱导剂或成骨诱导剂作用下 ,对原代和第 2代兔 MSCs进行成脂和成骨诱导培养 ,并鉴定成脂及成骨表型。结果表明 :原代及第 2代兔 MSCs均有一定的成脂、成骨能力 ,且第 2代细胞的分化能力较原代低。在诱导培养条件下 ,原代及第 2代兔 MSCs均能分化 ,成脂诱导 2 1d,75 %的兔 MSCs转化为脂肪细胞 ;成骨诱导 2 1d,75 %的兔 MSCs转化为成骨细胞。兔

Rabbit bone marrow derived mesenchymal stem cells(MSCs)are multipotent.We studied the adipogenic and osteogenic differentiation poteint using adipogenic supplement (AS) or osteogenic supplement (OS) in vitro. Specific markers of this induced adipogenic and osteogenic lineage were identified. The findings showed that the rabbit MSCs are capable of differentiating into adipogenic and osteogenic lineages spontaneously. On the 21th day, approximately 75% rabbit MSCs were induced to adipogenic or osteogenic cells in medium containing AS or OS, respectively. These results demonstrated that the differentiation of MSCs could be regulated in vitro. The underlying molecular mechanisms of adipogenic or osteogenic differentiation await elucidation.