XW630对大鼠成骨细胞雌激素受体基因表达的影响

Regulation of XW630 on Estrogen Receptor Messenger RNA Expression in Osteoblasts

探讨抗骨质疏松新化合物 XW6 30促进成骨作用的机理。从 SD大鼠颅骨中分离培养成骨细胞 ,首次采用具有高灵敏度的逆转录 -聚合酶链反应 (RT- PCR)技术直接从大鼠成骨细胞中检测雌激素受体 m RNA的表达。结果表明 ,XW6 30具有促进成骨细胞 ERm RNA表达的作用 ,且呈时间依赖性。在相同浓度 (10 - 6 mol/ L)条件下 ,XW6 30作用强于四环素加哌嗪雌酮及单纯雌酚酮。XW6 30可能通过对

This study was performed to investigate the mechanism of an anti osteoporosis new drug XW630 for promoting the osteogenic action. Osteoblast clone was cultivated from SD adult rat calvaria in vitro, the estrogen receptor messenger RNA(ERmRNA) expression in the osteoblasts of rats was detected directly with high sensitive RT PCR firstly. The results indicated that XW630 can significantly promote ERmRNA expression in osteoblasts in the time dependent manner.Being superior to other two kinds of estrogen in the same concentration (10 -6 mol/L), XW630 probably plays an important role in the bone pathogenesis by means of ER gene regulatory functions.