氧等离子体硫化法制作可变形聚二甲基硅氧烷薄膜培养基底

Oxygen Plasma-vulcanized Deformable Polydimethylsiloxane Sheet Culture Substrates

发展了氧等离子体硫化法制作可变形聚二甲基硅氧烷薄膜培养基底的技术。与传统的加热硫化法相比 ,该法制作的基底表面具有亲水性 ,细胞的黏附、铺展以及基底皱褶变形的发展均非常迅速。此外 ,观察了细胞松弛素 D处理过程中基底皱褶的变化 。

A method of preparing deformable polydimethylsiloxane sheet culture substrates by oxygen plasma vulcanization was developed. As compared with the traditional heating vulcanization method,the substrates prepared in this way have hydrophilic surfaces, the adhesion and spreading of cells both occur quickly, and the wrinkling deformation of substrates develops quickly, too. In addition, the changes of wrinkles during treatment of cytochalasin D were observed, and the result shows that this technique has high temporal resolution.