中药甘黄注射液在异种排斥反应中作用初探

Preliminary Study on Effect of Ganhuang Injection on Reject Reaction of Xenograft

目的 :研究中药甘黄注射液在异种排斥反应中的作用。方法 :通过RT PCR技术检测甘黄注射液对猪血管内皮细胞 (porcineendothelialcells,PECs)激活相关基因表达的影响及采用51Cr释放法检测其对NK细胞杀伤与粘附作用的影响。结果 :未灭活人血清及人NK 92细胞可以上调猪血管内皮细胞E selectin和IL 1α基因的mRNA表达 ,具有激活PECs作用 ;甘黄注射液可抑制人血清处理和NK细胞处理的PECs激活相关基因的表达。细胞毒实验结果认为 ,该中药也可以抑制NK细胞对PECs细胞毒作用 ,而且具有剂量依赖关系 ,这与该药抑制NK细胞对PECs的粘附作用是一致的。结论 :甘黄注射液不仅可抑制超急性排斥反应中血清对内皮细胞激活作用 ,而且可抑制延迟性排斥反应中NK细胞的作用。

Objective: To study the effect of Ganhuang Injection (GHI) on reject reaction of xenograft. Methods: RT PCR technique was used to detect the activated relevant gene expression in porcine endothelial cells (PEC), and 51 Cr releasing method was used to test the killing and adhesion action of NK cells. Results: The normal human serum and human NK 92 cell could up regulate the mRNA expressions of E selectin and IL 1α gene in PEC, showing the PEC activating action, GHI could inhibit these activated gene expressions. Cyto toxic experiment showed that GHI could also inhibit the cytotoxicity of NK cell on PEC dose dependently, which was in accord with its inhibition on adhesive action of NK on PEC. Conclusion: GHI could inhibit not only the PEC activation in hyperacute rejection, but also the function of NK cells in delayed xenograft rejection.