实美橙子叶培养和植株再生

COTYLEDON CULTURE AND PLANTLET REGENERATION IN CITRUS SINENSIS

以实美橙子叶为外植体,在含2.4-D 1.0mgL^(-1),6-BA 0.5mgL^(-1)的MT 培养基上诱导愈伤组织,并将其在相同的培养基上继代增殖。在附加IBA 0.5～1.0mgL^(-1),6-BA 1.5～3.0mgL^(-1)的MT 分化培养基上培养近1月后,愈伤组织分化产生大量不定芽,长至2.0cm高时,在含NAA 0.5L^(-1),KT 0.05~mgL^(-1)的MT 生根培养基中诱导成完整的再生小植株。

Prolific white loose calli were induced from the cotyledon explants of Citrus sinensisOsbesk(cv.Shi Mei Cheng)cultured on Murashige and Tucker(MT)medium supple-mented with 2,4-D 1.0 mg/1 and 6-BA 0.5 mg/1,and subcultured on the same MT me-dium.When transferred to an MT medium supplemented with IBA 0.5～1.0 mg/1 and6-BA 1.5-3.0mg/1 and cultured for a month,these calli produced numerous adventitiousbuds.Developing shoots 2.0 cm in height were excised from the calli,and rooted readily onMT medium with the addition of NAA 0.5mg/1,kinetin 0.05 mg/1 and glycerine 2%.The rooted plantlets,when transplanted onto potting compost and kept in a greenhouse,survived well.