一氧化氮吸入对肺动脉高压大鼠血压和血浆中血管活性物质水平的影响

The Plasma Concentrations of Endothelin,Atrial Natriuretic Peptide and Calcitonin Gene Related Peptide and Blood Pressure in Rats with Pulmonary Hypertension Influenced by Exogenous Nitric Oxide

为探讨一氧化氮吸入是否对肺动脉高压的形成和发展具有阻抑作用 ,并进一步探讨血浆中血管活性物质与肺动脉高压形成和发展的关系。将 10 9只大鼠 (实验终结时 10 1只 )分为四组 :对照组腹腔注射生理盐水 ,其它三组腹腔注射野百合碱复制肺动脉高压模型 ,其中预防组同时吸入一氧化氮 ,治疗组第 3周才开始吸入一氧化氧 ,模型组自由呼吸。每隔 1周分别检测血浆中内皮素、心房钠尿肽和降钙素基因相关肽水平 ,测定右心室收缩压和颈动脉平均压。结果发现 ,腹腔内注射野百合碱时右心室收缩压由 1.71± 0 .35kPa升至 2 .35± 0 .4 4kPa ,第 3周末升至 4 .5 6± 0 .6 5kPa ,证明肺动脉高压已经形成。内皮素和心房钠尿肽均随肺动脉压力升高而增加 ,内皮素由 130± 4ng L升至 187± 37ng L ;心房钠尿肽由 172± 2 8ng L升至 2 0 7± 2 7ng L。降钙素基因相关肽则下降 ,由 2 32± 5 7ng L降至 187± 2 3ng L。间断长期吸入一氧化氮后 ,至第 5周末 ,内皮素和心房钠尿肽呈下降趋势 ,前者由模型组的 6 36± 14 8ng L降至预防组的 5 34± 4 4ng L ,后者由模型组的 1111± 2 4 8ng L降至预防组的 772± 14 5ng L ;降钙素基因相关肽则有所升高 ,由模型组的 4 5± 11ng L增至预防组的 80± 16ng L。右心室收缩压则下降 ,由

Aim To observe whether the level of endothelin (ET), atrial natriuretic peptide (ANP) and calcitonin gene related peptide (CGRP) in the plasma of rats with pulmonary hypertension (PH) can be influenced by exogenous nitric oxide. Methods A monocrotaline (MCT) PH model was founded in rat. Exogenous NO were inhaled during the forming and developing of PH. The changes of ET, ANP and CGRP and systolic pressure of right ventricle (RVSP), mean cervical arterial pressure were measured. Results It demonstrated that the RVSP of MCT rats had been elevated since the first week: from 1.71±0.35 kPa to 2.35±0.44 kPa, and (PH) was formed at the time of the third week: 4.56±0.65 kPa, the plasma concentration of ET and ANP in MCT rats were increased along with pulmonary arterial pressure, ET: from 130±4 ng/L, elevated to 187±37 ng/L; ANP: from 172±28 ng/L elevated to 207±27 ng/L at the first week, but CGRP were lowered: from 232±57 ng/L reduced to 187±23 ng/L at the first week. After exogenous NO were inhaled intervally at long term, ET and ANP have the tendency to reduce: ET reduced from 636±148 ng/L to 534±44 ng/L; ANP reduced from 1 111±248 ng/L to 772±145 ng/L. But CGRP increased from 45±11 ng/L to 80±16 ng/L at the fifth week, RVSP lowered from 2.35±0.44 kPa to 2.02±0.29 kPa at the first week. In comparison with control group, there is a significant difference (P<0.05). Conclusions There is a positive significant correlation between the forming and developing of PH and plasma concentration of ET and ANP, but negative significant correlation with CGRP. The methods of interval and long term time of inhaled exogenous NO could not only attenuate the forming of PH and partly prohibit the process and progress of PH, but also had an effect on the formed PH.