冠状动脉搭桥术患者不同组织中几种血管活性肽的含量

Level of Peptides Endothelin?Angiotensin Ⅱ,Calcitonin Gene Related Peptide and Atrial Natriuretic Polypeptide in Different Tissues of Coronary Artery Bypass Graft Patients

为探讨血液中重要多肽因子内皮素、血管紧张素Ⅱ及降钙素基因相关肽与心房钠尿肽在冠状动脉搭桥术患者不同组织中的含量及其意义。选择施行冠状动脉搭桥术患者 32例 ,术中取得病理心包、心耳组织及心包液以及静脉血。组织匀浆后取离心上清液 ;静脉血经抗凝、离心、取血浆 ,用放射免疫分析法测定冠状动脉搭桥术患者血浆及心包、心包液、心耳中的内皮素、血管紧张素Ⅱ、降钙素基因相关肽及心房钠尿肽含量。结果发现 ,血浆与心包液中的内皮素 1、血管紧张素Ⅱ及降钙素基因相关肽含量存在明显的差异 ,心包与心耳中的内皮素 1、血管紧张素Ⅱ及降钙素基因相关肽含量也存在明显差异。血浆与心包液中的心房钠尿肽无明显差别 ,而心包与心耳中的心房钠尿肽则存在明显的差异。结果提示 ,冠状动脉搭桥术患者血浆内皮素、降钙素基因相关肽、血管紧张素Ⅱ及心房钠尿肽均会出现明显的变化。

Aim To investigate the changes of endothelin (ET),calcitonin gene related peptide (CGRP), angiotensin Ⅱ (AngⅡ) and atrial natriuretic polypeptide (ANP) in plasma of patients underwent coronary artery bypass graft (CABG). Methords ET, CGRP, Ang Ⅱ and ANP in plasma of 32 patients with coronary heart disease who had the CABG operation were studied. The pericardium, auricle, pericardial fluid were got during the CABG operation. The tissue were homogenated, centrifuged and the supernatant was got; anticoagulant blood were centrifuged and the plasma was got then the ET, AngII, CGRP and ANP in the luquid were assayed by RIA. Results ET in the pericardial and auricle were very different (P<0.001). AngⅡ in the plasma and pericardial fluid were obviously different, and the same as that in the pericardium and auricle (P<0.001). CGRP in the plasma and pericardial fluid, pericardium and auricle, were also different (P<0.05,P<0.001). Though there was no difference of ANF between plasma and pericardial fluid, ANF in the pericardium was higher than in the auricle (P<0.001). Conclusion Different tissue of CABG patients had alternated ET,CGRP,AngⅡ and ANP.