抑癌基因蛋白P33^(ING1)和VEGF在大肠癌中的表达

Study on the Expression of P33^(ING1) and Vascular Endothelial Growth Factor in Colorectal Carcinoma

目的 研究血管内皮生长因子 (VEGF)和抑癌基因蛋白P33ING1在大肠癌组织中的表达、相互关系及肿瘤生物学行为的相关性。方法 对 5 4例大肠癌患者的手术切除之癌组织和癌周正常组织 ,应用免疫组织化学方法检测其VEGF和抑癌基因蛋白P33ING1的表达情况 ,探讨两者之间的相互关系及其与肿瘤生物学行为的相关性。结果 VEGF在正常组织中呈低表达 ,在肿瘤组织中呈高表达 ;P33ING1在正常组织中呈高表达 ,在肿瘤组织中呈低表达。P33ING1与VEGF的表达呈高度负相关性 (P <0 .0 1) ,并与组织类型、组织分化程度、淋巴结转移、临床分期和 5年生存率相关 (P <0 .0 5 )。结论 抑癌基因蛋白P33ING1在大肠癌中表达水平降低 ,可能是癌肿发生、生长和浸润转移的重要因素 ,可作为判断肿瘤恶性程度的重要指标。

Objective To investigate the expression of VEGF and P33 ING1 and the relationship between these two proteins, as well as the relationship beween the expressions of these two proteins and the biological behavior of carcinoma. Methods Immunohistochemical method was used to detect the expression of VEGF and P33 ING1 in the carcinoma tissues and normal tissues around the foci. The relationship between these two proteins, together with the relationship between the expression of these two proteins and the biological activity of carcinoma was further investigated. Results The expression of VEGF was high in carcinoma tissues while low in normal tissues. As for P33 ING1 , the case was to the contrary. There was correlation between the expressions of these two proteins. And the expression of these two protein was related to the tissue type, tumor differentiation, metastasis of lymph node, Dukes stage and five-year survival. Conclusion The low expression of P33 ING1 in carcinoma may be important factors related to carcinogenosis, development, invasion and metastasis, which can be used as predictors of malignant behavior of colorectal carcinoma.