NF-κB信号通路在失血性休克缺血再灌注肺组织中的变化

Changes of NF-κB signal transduction pathways in lung tissues of ischemia-reperfusion injury following hemorrhagic shock

目的 :探讨核因子 κB(NF κB)信号通路在失血性休克缺血 再灌注 (I/R)损伤肺组织中的变化及意义。 方法 :建立失血性休克I/R家兔模型。采用原位杂交、免疫组化结合图像分析以及酶联免疫吸附试验 (ELISA)检测实验动物I/R肺组织损伤前后I κB激酶 β(IκK β)mRNA表达和NF κB的活性以及支气管肺泡灌洗液 (BALF)中肿瘤坏死因子 α(TNF α)、白细胞介素 6 (IL 6 )的含量变化。并进行肺组织病理学光镜检查。 结果 :模型组上述指标显著升高 (P均 <0 .0 1) ;I/R损伤动物肺组织明显呈炎症病理改变。 结论 :IκK β激活 /NF κB核移位 /细胞因子释放在失血性休克I/R并发急性肺损伤 (ALI)的病理机制中发挥重要作用。

Objectives:To investigate the changes and significance of NF κB signal transduction pathways in lung tissues of ischemia reperfusion(I/R) injury following hemorrhagic shock. Methods:Rabbit model of ischemia reperfusion injury following hemorrhagic shock was used in this study. The expressions of IκK β and NF κB in the lung tissues and the concentration of tumor necrosis factor α(TNF α) ,Interleukin 6(IL 6) in bronchoalveolar lavage fluid(BALF) were measured by in situ hybridization, immunohistochemistry combined with in situ quantitative analysis and enzyme linked immune adsorbing analysis (ELISA), respectively. And the pathological changes were examined with light microscope in lung tissues. Results:In I/R injury group, the expressions of IκK β was 0.201±0.026 and NF κB was 0.169±0.023 in lung tissues. The levels of TNF α(835.82±112.39 ng/L)and IL 6(1 232.78±153.28 ng/L) in bronchoalveolar lavage fluid (BALF) were obviously higher than those in normal group 〔 IκK β 0.032±0.004,NF κB 0.035±0.005, TNF α (201.52±38.78)ng/L,IL 6 (127.81 ±15.28 ) ng/L〕(all P <0.01). And the severity of lung tissue damages increased in I/R injury following hemorrhagic shock. Conclusions: The IκK β expression, NF κB activation and cytokine secretion may play an important role in the inflammatory reaction of lung tissues I/R injury following hemorrhagic shock.