激素对水稻花药培养力的影响研究

STUDIES ON THE EFFECT OF DIFFERENT HORMONE TREATMENTS ON RICE ANTHER CULTURE

在 M_8诱导培养基申附加适当的2,4-D、NAA、Kt、6-BA 和4pu-30等激素,对籼粳、籼籼交水稻 F_1的花药进行培养。结果表明,在8种处理中,以2.4-D 2mg/L 加 NAA 2mg/L、Kt 1mg/L、6-BA 0.5mg/L 对籼粳交材料平均愈伤组织(7.7%)虽略低于其它5个处理(8.94%～11.88%),但绿苗分化率最高(17.74%)。对籼籼交水稻的出愈率(0.61%)虽低于其它两个处理(13.5%～15.3%),但绿苗分化率(50%)和花药培养力(0.3%)都明显超过其它处理(分别为0～7.69%和0～0.1%)。2,4-D 与 NAA 配合附加不同的细胞分裂素分别比单独用2,4-D 或2,4-D配 Kt 的处理的花药培养力高.附加激动素对愈伤组织的发生有抑制作用,但能提高绿苗分化率.6-BA 与激动素的作用相似。4pu-30与2,4-D、NAA 配合,有利于愈伤组织的产生,但有降低其质量的趋势,且与其它细胞分裂数有拮抗作用。

Anthers of the F_1s from crosses of indica x indica and indica x japonica were cultured on M_8 medium supplemented with 2,4-D,NAA,Kt,6-BA,4pu-30 or other hormones. 2,4-D 2mg/l combined with NAA 2 mg/l or Kt 1 mg/l or 6-BA 0.5 mg/l gave the highest green plantletinduction rate(17.74%)for indica x japonica crosses,although their average callus induction rat(7.7%)was slightly lower than that of the other five hormone combination treatments(8.94%～11.88%).For indiea x indica crosses,their green plantlet induction rate(50%)and anther culture ability(0.3%)were higher than the other treat- ments(0～7.69% and 0～0.1%,respectively),though their callus induction was low (0.61%)as compared to other treatments(13.5%～15.3%).Anther culture ability was higher in treatments of 2,4-D combined with NAA supplemented with different cytokinins than in those of 2,4-D alone or 2,4-D combined with Kt.Addition of Kt depressed the in- duction of calli but enhanced green plantlet induction rate.6-BA showed a similar effect as Kt.4pu-30 combined with 2,4-D or NAA promoted the induction of calli but had a nega- tive effect on their quality,and appeared to be antagonistic to other cytokinins.