摘要
试验了不同浓度的氯化钙(CaCl_2)、硝酸银(AgNO_3)、氯化胆碱(CC)及脱落酸(ABA)对籼稻花药愈伤组织诱导率和继代效果,发现在培养基中含有600mg/L CaCl_2、10mg/L AgNO_3、300mg/L CC均能提高愈伤组织诱导率,在继代培养中加快细胞的生长,有利于胚胎发生。ABA对细胞胚性的发生、恢复和保持有良好的效果。
The effects on callus induction and subculture of indica rice anther culture were tested by using different concentration of calcium chloride (CaCl2. 2H2O), silver nitrate(AgNO3), Choline chloride (CC) and abscisic acid (ABA). It was found that they enhanced callus induction frequency and increased cell growth in subculture when the culture medium contained 600mg/L of CaCl2. 2H2O, 10mg/L of AgNO3 and 300mg/L CC. ABA has a better effect on restoring and maintaining embryonic cell character
出处
《西南农业学报》
CSCD
1992年第2期28-32,共5页
Southwest China Journal of Agricultural Sciences
关键词
籼稻
花药培养
培养基
诱导率
Indica rice Anther culture Induction frequency Subculture Medium
