亚洲稻(Oryga Sativa L.)分类的酯酶同工酶研究——兼论水稻酯酶同工酶基因表达的时空性

CLASSIFICATION OF ASIAN RICES BY ESTER ASE 1SOZYMES

对1270多份亚洲栽培稻各生态型地方品种及普通野生稻的分析表明,鉴别籼、粳及野生稻用萌动期种子检测酯酶同工酶效果较好,可出现16条酶带,其中10A为粳稻主特征带,11A为籼稻的主特征带,13A为Aus及一部分西南亚籼的主特征带,14A为普通野生稻的主特征带。10A、11A、13A、14A与栽培稻起源及籼粳分化密切相关,可作为区分籼粳的指标。本文还对国内外各研究者的酯酶同工酶分析结果进行了对比。

A survey of esterase variation by polyacrylamide gel was carried out in more than 1270 accessions of Asian cultivated and wild rices. It was found that seeds soaked for 24 hours at 37℃ gave the best results. Among the sixteen isozyme bands numbered from anode to cathode, 10A, 11A, 13A, and 14A were the discriminating bands of the Joponica (Keng) , Indica (Hsien), Aus (including Javanica), and wild rices, respectively.It has been shown before that the four bands are coded presumably by alleles of a new gene for esterase, and they are involved in the evolution and differentiation of Asian cultivated rice.