改构型和野生型aFGF对肠缺血-再灌注损伤后肝肾功能的影响

Effects of acidi fibroblast growth factor on hepatic and renal functions after intestinal ischemia/reperfusion injury

目的 观察肠缺血再灌注损伤后的肝、肾功能的变化 ,并探讨不同类型酸性成纤维细胞生长因子 (a FGF)对肠缺血再灌注损伤后的肝、肾功能的保护作用。方法 将 78只 Wistar大鼠分成假手术组 (C)、生理盐水治疗组 (R)、改构型 a FGF治疗组 (rh F)和野生型 a FGF治疗组 (wt F) ,后 3组根据再灌注时间又分为 2、6、12和 2 4 h4个时间点。阻断肠系膜上动脉 4 5 m in后松夹制备肠缺血再灌注模型。于不同时间点腹主动脉抽血 5 ml,检测肝、肾功能的变化。结果 与 C组比较 ,R组、rh F组和 wt F组肝、肾功能都有明显下降 ,以 R组下降更明显 ,rh F组次之 ,wt F组肝、肾功能恢复得最好。组织病理学检查发现 ,3组动物均有小肠绒毛脱落、黏膜下层炎细胞浸润等改变 ,其严重程度与肝、肾功能指标水平一致。结论 肠缺血再灌注损伤导致多脏器功能损伤 ,野生型、改构型 a FGF对脏器功能有明显的保护作用。

Objective To investigate the change in hepatic and renal functions parameters after intestinal ischemia-reperfusion(I/R) injury, and to explore the effects of acidic fibroblast growth factor(aFGF) on hepatic and renal functions after intestinal I/R injury in rats. Methods Seventy-eight Wistar rats were divided into four groups, which are sham-operated(C) group, ischemia (45 minutes) plus reperfusion (R), reconstructive human aFGF treatment (rhF), and wild type aFGF treatment (wtF) groups. The animals were sacrificed at 2, 6, 12 and 24 hours, respectively. Hepatic and renal functions was analyzed. Results In comparison with those in group C, the hepatic and renal functions were damaged in group R, rhF and wtF decreased. Treatment with rhF and wtF markedly abated the hepatic and renal dysfunction. The desquamation of intestine villi and infiltration of inflammation cells in the submucosa were observed in all groups. Conclusion Hepatic and renal functions are damaged after intestinal I/R injury. Treatment with rhF and wtF could protect against multiple organ dysfunction associcated with intestinal ischemia- reperfusiun injury.