甘蓝型油菜显性细胞核雄性不育基因的AFLP标记

Identification of AFLP Markers Linked to the Dominant Genic Male Sterility Gene in Brassica napus L.

用甘蓝型油菜双基因显性细胞核雄性不育系Rs10 4 6A和欧洲油菜品种Samourai构建了一个回交分离群体。在群分法 (BSA)构建的不育池和可育池中共筛选了 2 5 6对AFLP引物组合 ,找到了与不育基因紧密连锁的两个AFLP标记(EA0 3MC15 99和EA0 7MC0 12 35) ,它们与不育基因的遗传图距分别是 3.5cM和 5 .5cM ,而且位于不育基因的同一侧 ,标记间相距 2 .0cM。这两个标记的发掘 ,对运用分子标记辅助选择技术来改良显性细胞核雄性不育两型系及其恢复系具有重要意义。

One backcross population was constructed from the dominant genic male sterile line Rs1046A in Brassica napus L. and the rapeseed cultivar Samourai. Sterile bulk(BS) and fertile bulk(BF) prepared from above population using BSA strategy were subjected to AFLP analysis. A total number of 256 primer combinations were used and two markers(EA03MC15_ 99 and EA07MC01_ 235 ) tightly linked to the dominant genic male-sterile gene( Ms ) were identified, with a map distance of 3.5 cM and 5.5 cM, respectively. These two markers, 2.0 cM away from each other and residing at the same side of Ms, would play an important role in the genetic improvement of this GMS line through marker aided selection(MAS).