原位RT-PCR方法检测妊高征患者胎盘内皮型一氧化氮合酶-mRNA的表达

In situ RT-PCR localization and quantitative detect the expression of Endothelial nitric oxide synthase-mRNA in the placentae of pregnancy-induced hypertension syndrome patients

目的 :检测内皮型一氧化氮合酶 -mRNA(eNOSmRNA)在妊高征患者胎盘组织中的定位和表达水平的变化。方法 :采用标记引物的直接原位RT -PCR方法检测eNOSmRNA在40例妊高征患者与30例正常足月孕妇胎盘组织的定位和表达 ,并利用IMAGER -PROPLUS图像分析处理系统对eNOSmRNA在妊高征与正常足月胎盘合体滋养细胞表达水平进行定量分析。结果 :eNOSmRNA主要定位在正常足月孕妇和妊高征患者胎盘绒毛的合体滋养细胞。妊高征患者胎盘绒毛合体滋养细胞eNOSmRNA阳性染色减弱 ,而干绒毛和终末绒毛血管内皮细胞eNOSmRNA阳性染色增强。定量分析表明 ,妊高征患者胎盘合体滋养细胞eNOSmRNA表达低于正常足月孕妇。结论 :eNOSmRNA主要定位在胎盘绒毛的合体滋养细胞 ,妊高征患者胎盘绒毛合体滋养细胞eNOSmR

Objective:To study the localization and the change of expression level of enˉdothelial nitric oxide synthase mRNA(eNOSmRNA)in the placentae of Pregnancy-induced hypertension syndrome(PIH).Methods:Frozen section of placental tissue of40PIH and30normal term pregnant woman were studied.The localization and expression of eNOSmRNA were measured by primer-labeled in situ RT-PCR,IMAGE-PRO PLUS System image the level of eNOSmRNA expression in syncytiotrophoblast.Results:The positive staining of eNOSmRNA were all evident in syncytiotrophoblast in normotensive and PIH.The positive staining of eNOSmRNA in syncytiotrophoblast were weaker in PIH when compared with norˉmotensive,but the positive staining of eNOSmRNA in endothelial cells of the stem villous vessels in PIH were greater than normotensive.By quantitative analysis,the level of eNOSmˉRNA expression in syncytiotrophoblast was decreased significantly in patients with PIH when compared with those of normal pregnant term woman.Conclusion:The expression of eNOSmˉRNA is mainly present in syncytiotrophoblast,the lower of eNOSmRNA expression in syncyˉtiotrophoblast may be a importmant factor of the pathogenesis of PIH.