人参皂甙单体Rb_1对大鼠在体心脏收缩性能的影响(英文)

Effect of ginsenoside Rb_1 on the contractility in rat heart

背景:作者前期实验以左室内压及其最大升降速率等8项心肌力学与血流动力学参数为指标,观察到人参二醇和三醇组皂甙均能使离体大鼠工作心脏收缩性能减弱。目的:探讨人参皂甙单体Rb1对大鼠心率、动脉压、左室内压(leftven-tricularpressure,LVP)、左室舒末压(leftventricularend-diastolicpres-sure,LVEDP)、室内压最大上升与最大下降速率(maximalrisingandfallingrateofventricularpressure,±dp/dtmax)6项指标的影响。设计:完全随机、空白对照的双盲法实验。地点、材料和干预:在吉林大学基础医学院动物实验室完成本实验,室温200C。Wistar大鼠购于吉林大学动物部,雌雄不拘。按随机抽签法将动物分为5组,即对照组、Rb195mg/kg组、Rb1190mg/kg组、Rb1285mg/kg组、维拉帕米(异搏定)36mg/kg组。在Wistar大鼠在体心脏上观察给予大鼠Rb195,190,285mg/kg,维拉帕米36mg/kg对大鼠心率、动脉压、LVP、LVEDP,+dp/dtmax,-dp/dtmax6项指标的影响,并与对照组进行比较。整个过程由高级实验师操作完成。主要观察指标:静脉注入维拉帕米与95,190,285mg/kg浓度Rb1后大鼠心率、动脉压、LVP、LVEDP,+dp/dtmax,-dp/dtmax6项指标的比较结果。结果:Rb1浓度在95,190,285mg/kg范围内均使心率、动脉压、左室收缩压、左室舒张压、室内?

BACKGROUND:According to hemodynamic parameters such as left ventricular press ure and maximal rising and falling rate of ventricular pressure(± dp/dtmax) of rat,the author observed that panaxadiol and panaxatriolsaponin could inhibit th e contractility of isolated hearts. OBJECTIVE:To observe the effect of ginsenoside Rb1 on heart rate(HR),arterial pressure(AP),left ventricular systolic pressure(LVSP),left ventricular end- di astolic pressure(LVEDP),andmaximal rising and falling rate of ventricular pressu re(± dp/dtmax) of rats. DESIGN:Totally randomized blank controlled double- blind trials. SETTING AND ANIMALS:Healthy Wistar rats weighing 250-300 g, male and female half to half,were purchased from the Animal Laboratory of School of Basic Medic al Sciences,Jilin University. INTERVENTION:The rats were randomly divided into 5 groups:control group,Rb1 g roup(95 mg/kg),Rb1 group(190 mg/kg),Rb1 group(285 mg/kg) group and verapamil gro up(isoptin)(36 mg/kg) to observe the effect of ginsenoside of different doses on HR,AP,LVSP,LVEDP and ± dp/dtmax, and verapamil 36 mg/kg in Wistar rat heart.Th e whole procedure was operated by senior technicians. MAIN OUTCOME MEASURES:HR,AP,LVSP,LVEDP,± dp/dtmax. RESULTS: Rb1(in the range of 95 mg/kg,190 mg/kg,and 285 mg/kg) could decrease HR,AP,LVSP,LVEDP,and ± dp/dtmax in a dose- dependent manner.LVSP decreased from[(6.65± 2.30 ) kPa](95 m g/kg),[(5.32± 1.90) kPa](190 mg/kg) to[(5.00± 1.50) kPa](285 mg/kg) in adminis tration group compared with control group[(312.00± 25.00) kPa].The ± dp/dtmax of administration groups decreased remarkably from[(305± 23) kPa](95 mg/kg) to[(252.5± 30 ) kPa](285 mg/kg),from[(166.25± 18) kPa](95 mg/kg) to[(146± 15.60) kPa](285 mg/kg).Similar results were observed when Ca2+ blocker verapamil(36 mg/kg) was administrated. CONCLUSION: Rb1 can inhibit contractility of the myocardium,and its mechanism is related to Ca2+ channel.