新生儿缺氧缺血性脑病血浆一氧化氮及一氧化氮合成酶水平对预后判断的意义(英文)

Significance of the changes of serous nitric oxide and nitrogen monoxide synthase levels of neonatal hypoxic-ischemic encephalopathy in prognosis judgments

目的:探讨新生儿缺氧缺血性脑病(hypoxic-ischemicencephalopathy,HIE)时一氧化氮、一氧化氮合成酶(Nitrogenmonoxidesynthase,NOS)水平的变化及其对预后判断及恢复期指导的意义。方法:采用比色等方法测定32例HIE患儿及30例正常新生儿血浆一氧化氮、一氧化氮合成酶活性水平。结果:正常对照组一氧化氮、NOS水平分别为(72.84±12.35)μmol/L和(0.203±0.05)kU/g,HIE组为(129.80±32.66)μmol/L和(0.352±0.124)kU/g;其中轻度HIE为(105.65±36.30)μmol/L和(0.289±0.09)U/mg;中度HIE为(135.03±24.40)μmol/L和(0.325±0.16)kU/g;重度HIE组为(144.55±26.32)μmol/L和(0.364±0.01)kU/g。HIE急性期一氧化氮、NOS水平校正常新生儿明显升高(t=-8.97,P<0.01),中、重度HIE升高更显著(t=-10.62,-12.01,P<0.01),中、重度HIE患儿较轻度HIE患儿升高明显(t=-2.12,-2.92,P<0.05,P<0.01)。中、重度HIE之间则无明显差别。结论:一氧化氮、NOS水平高低与HIE患儿脑损伤程度和预后有关,可作为判断HIE患儿病情恢复及预后的指标之一。

AIM:To discuss the level changes of nitric oxide(NO) and nitrogen monoxide sy nthase(NOS) in neonatal hypoxic- ischemic encephalopathy (HIE) and its signific ance on prognosis judgments and restoration supervision. METHODS:Color matching method, and etc. were introduced in detecting the sero us NO and NOS levels of 32 HIE cases as well as 30 normal neonates. RESULTS:The NO and NOS levels of the control group were(72.84± 12.35)ì mol/ L and(0.203ì mol/L and(0. 352ì mol/L and(0.2 89ì mol/L and(0.325HIE cases, and (144.55ì mol/L and (0.364-8.97, P<0.01), while were more s ignificantly higher in moderate and severe HIE cases (t=-10.62,-12.01, P<0. 01). There was significant difference between slight and moderate, severe HIE c ases (t=-2.12,-2.92,P< 0.05,P< 0.01),but there was no significant difference between moderate and severe HIE cases. CONCLUSION:The levels of NO and NOS relate to the degree of the cerebral dama ges in HIE patients as well as the prognosis,which can be used as one of the ind ices in the judgment of the restoration and prognosis in HIE patients.