摘要
利用ANSYS软件对毛细管电泳芯片微沟道内样品流动情况进行模拟 ,获得了不同进样模式下微沟道的结构与流体流速之间的关系 ,对芯片整体结构参数进行设计 :毛细管微沟道最终尺寸为宽度 16 μm ,深度 10 μm ,有效分离长度为 3.5cm的圆角转弯形沟道。采用激光诱导荧光原理进行实验测试 ,建立测试系统 ,对两段不同长度的DNA片段实现了基线分离 。
The fluid distribution in the micro channel of ICEC ( Integrated Capillary Electrophoresis Chip) was simulated using ANSYS software. The relations between the micro channel structure and flow velocity under different sample injection modes were obtained. Then the optimized channel structure was chosen by the width of 16μm , the depth of the chip was determined 10μm and the effective separation length of 3.5cm with round corner channel. The chip structure was designed. Using the principle of laser induced fluorescence, the detection system for this chip was set up. The experiment for the ICEC could realize the base line separation of two DNA restriction fragments ranging from 500bp to 1021bp with excellent resolution. The result of the experiment would be the stable groundwork for further research in ICEC.
出处
《微纳电子技术》
CAS
2003年第7期340-343,共4页
Micronanoelectronic Technology
基金
哈工大跨学科交叉基金资助项目 (HIT MD2 0 0 0 0 3 )
