肌苷对海人藻酸引起纹状体损伤的保护作用

Protective effect of inosine on intrastriatal kianic acid injection induced lesion in the rat

目的 :建立大鼠纹状体内注射海人藻酸 (KA)模型 ,并观察肌苷对纹状体损伤面积及NADPH脱氢酶 (NADPH d)阳性神经元数目的影响 .方法 :采用纹状体内注射KA制备模型 .1 0只大鼠分成 2组 (n =5 ) ,分别于手术前 1d给予肌苷或等量生理盐水 (对照组 ) ,腹腔注射 ,每日 3次 ,持续给药至手术后第 3日结束 ,利用尼氏染色和NADPH d的组织化学方法 ,观察肌苷组和对照组纹状体损伤面积及NADPH d阳性神经元数目的差别 .结果 :肌苷可以使KA引起的纹状体损伤面积减小并增加NADPH d阳性神经元的数目 .结论 :肌苷可以减轻兴奋性毒素KA引起的纹状体损伤 ,并对纹状体内的NADPH

AIM: To establish the animal model of intrastriatal kainic acid (KA) injection and to investigate the effect of systematic administration of inosine on the striatal lesion size and the number of NADPH d positive neurons in this model. METHODS: KA was stereotaxically injected into the left striatum of rats. Ten rats were divided into 2 groups: Inosine group ( n =5), receiving ip inosine administration, (50 g·L -1 , 75 mg·kg -1 ) , 3 times each day, from 1 day prior to operation to day 3 postoperatively when the rats were sacrificed, and control group ( n =5), receiving normal saline instead of inosine. Nissl stain and NADPH d histochemistry were then performed to investigate the difference of lesion size and NADPH d positive neurons between inosine and saline treated groups. RESULTS: Inosine decreased the size of KA induced striatal lesion area and increased the number of NADPH d positive neurons. CONCLUSION: Inosine can attenuate KA induced striatal lesion and show protective effect on NADPH d positive neurons in the striatum. These findings provide a pontential clinical use of inosine in the CNS disease.