中药肝复健冲剂对大鼠肝纤维化形成的影响

Influence of Ganfujian granule formula on process of liver fibrosis in rat

目的 :观测中药肝复健冲剂对二乙基亚硝胺 (DEN)诱导大鼠肝纤维化形成的影响 .方法 :建立DEN诱导大鼠肝硬化模型 ,同时给予肝复健冲剂进行干预 ,应用天狼红 苦味酸特染法、免疫组化染色法及计算机图像分析技术检测中药对DEN致大鼠肝纤维化过程胶原沉淀及肝细胞增殖特性的影响 .结果 :随着模型大鼠肝纤维化程度加重 ,肝内胶原沉积量亦呈逐渐增加趋势 .中药组大鼠肝脏炎性损害及肝纤维化程度均较同期模型组大鼠轻 ,胶原沉积量及细胞增殖核抗原(PCNA)阳性着色肝细胞数明显减少 ,两组间差异有统计学意义 ,P <0 .0 5 [胶原沉积量 :4wk(1 5 .2 0± 2 .2 1 )vs (7.1 9± 0 .97) ;1 2wk(2 7.1± 4 .6 )vs (1 6 .0± 1 .7) .阳性表达PCNA肝细胞数 :1 2wk(2 1 .1± 0 .32 )vs(6 .4± 0 .2 ) ;1 6wk (31 .1± 7.1 )vs (1 0 .1± 0 .8) ].结论 :中药肝复健冲剂可通过减少肝内胶原沉积及可能恶性增殖的肝细胞 ,抑制和延缓实验性动物肝纤维化的形成 。

AIM: To investigate the effect of ganfujian granule on collagen sediment in rat hepatic fibrosis induced by diethylnitrosamine (DEN). METHODS: DEN induced live fibrosis model was established and ganfujian (GFJ) granule was administrated as intervention measure. Influence (EFJ group) of ganfujian granule on collagen sediment and hepatocyte proliferation were examined by Picric acid Sirus red staining and immunohistochemical staing methods, and analyzed by computer image analysis technique. RESULTS: Collagen sediment in rat liver increased gradually with the exacerbation of hepatic fibrosis in the model. Inflammatory damage and the degree of liver cirrhosis were lower GFJ group than in model group. Collagen sediment in liver and positive expression of proliferotive cell number ontgen (PCNA) in hepatocytes were decreased significantly in GFJ group (collagen sediment: 4 wk (15.20± 2.21) vs (7.19±0.97);12 wk (27.1±4.6) vs (16.0± 1.7). positive expression of PCNA in hepatocytes: 12 wk (21.1 ±0.32) vs (6.4±0.2); 16 wk(31.1±7.1) vs (10.1±0.8) ( P <0.05). CONCLUSION: GFJ granule can inhibit and retard rats liver fibrosis by reduce collagen sediment in rat liver and may prevent liver cirrhosis transit to hepatocarcinoma in some degree by inhibit over proliferating of hepatocytes.