犬颅脑爆炸伤后脑组织NO含量的变化及L-NAME的脑保护作用

Alteration of NO content in brain tissue and the protective effect on brain of L-NAME after cranio-cerebral explosive wound

目的 探讨一氧化氮 (NO)在犬颅脑爆炸伤后继发性脑损害中的作用和N 硝基 L 精氨酸甲酯(L NAME)的脑保护作用。方法 模拟爆炸性武器致犬颅脑伤 ,L NAME组伤后 30分钟静脉给药 (5mg kg) ,伤后 1、3、6小时测定脑组织NO含量及水含量。结果 致伤组伤后 1、3、6小时NO含量显著升高 (P <0 .0 1) ,3小时达峰值。L NAME组伤后NO含量亦显著升高 (P <0 .0 5 ) ,但均明显低于同时相点致伤组水平(P <0 .0 5 )。致伤组伤后 1小时水含量升高非常显著 (P <0 .0 1) ,随时间延长逐渐升高 ,L NAME组各时相点水含量均明显低于致伤组 (P <0 .0 5 ) ,伤后 1小时与正常对照组比较无明显差异。结论 NO在颅脑爆炸伤后继发性脑损害过程中起重要作用 ,伤后 30分钟给予L NAME可降低脑组织NO含量 。

Objective To explore the role of nitric oxide(NO) in secondary injury of brain after cranio cerebral explosive wound and the protective effect of N nitro L arginine methyl ester(L NAME) on brain.?Methods Dogs were wounded by simulating explosive weapon,and L NAME was given 30 min after wounding(5mg/kg.iv). The content of NO and water of brain tissue were measured 1,3,and 6h respectively after wounding.?Results The content of NO in the wound group was significantly raised than that of the control group in the study period(P<0.01)and reached peak level 3h after wounding.The content of L NAME group also increased (P<0.01),but was significantly less severe than that of the wound group(P<0.05).The content of water in the wound group was significantly increased than that of the control group 1h after wounding(P<0.01)and it changed greatly as time went on. The content of water of brain tissue in L NAME group was significantly decreased than that of the wound group(P<0.05),and there was no difference compared with the control group 1h after wounding.?Conclusion NO plays an important role in the process of secondary injury of brain after cranio cerebral explosive wound.The use of L NAME 30min post wound may decrease the content of NO in brain tissue,and reduce brain edema.