摘要
Purpose: To study the feasibility of adeno-associated virus mediated gene transfection tocultured human iris pigment epithelium (IPE) cells in vitro.Methods: Recombinant replication deficient adeno-associated viruses (AAV) expressingLacZ gene were produced without helper virus. The LacZ gene was transduced into culturedhuman IPE cells.Results: Cultured human IPE cells stained positively anticytokeratin, The titer ofrAAV-LacZ was 2.1 × 108 virus particles/ml, 42% cultured human IPE cells expressedβ-galactosidase 7 days after transfection and 67% after 14 days.Conclusions: Recombined AAV produced without helper virus can transfer a foreign geneinto human IPE cells with high efficiency in vitro.
Purpose: To study the feasibility of adeno-associated virus mediated gene transfection to
cultured human iris pigment epithelium (IPE) cells in vitro.
Methods: Recombinant replication deficient adeno-associated viruses (AAV) expressing
LacZ gene were produced without helper virus. The LacZ gene was transduced into cultured
human IPE cells.
Results: Cultured human IPE cells stained positively anticytokeratin, The titer of
rAAV-LacZ was 2. 1×108 virus particles/ml, 42% cultured human IPE cells expressed
β-galactosidase 7 days after transfection and 67% after 14 days.
Conclusions: Recombined AAV produced without helper virus can transfer a foreign gene
into human IPE cells with high efficiency in vitro. Eye Science, 2003; 19: 49 - 53.
