摘要
Purpose: To investigate the effects on telomerase activity, human telomerase reversetranscriptase (hTERT) gene and TEP1 mRNA of retinal pigment epithelial (RPE) cells weretreated by TGF-β1 of different concentration.Methods: The cultured human RPE cells were treated with TGF-β1 at differentconcentration(0 ng/ml, 0. 01 ng/ml, 0.1 ng/ml, 1 ng/ml, 10 ng/ml) for 24 h, thentelomerase activity was detected by telomerase repeat amplification protocol(TRAP). Theexpression of hTERT mRNA and TEP-1mRNA were detected by reverse transcriptionpolymerase chain reaction(RT-PCR).Results: TRAP and RT-PCR showed when the concentration of TGF-β1 was graduallyincreased, telomerase activity and the expression of hTERTmRNA were gradually reduced,TEP1mRNA showed no apparent differential expression.Conclusion: TGF-β1 can down-regulate telomerase activity and the expression of hTERTmRNA, but no effection on TEP-1mRNA, hTERTmRNA expression was in accordance withtelomerase activity in hRPE cells, hTERT gene plays a crucial role in the expression oftelomerase activity, while TEP1 plays a much smaller role.
Purpose: To investigate the effects on telomerase activity, human telomerase reverse
transcriptase (hTERT) gene and TEPlmRNA of retinal pigment epithelial (RPE) cells were
treated by TGF-β1 of different concentration.
Methods: The cultured human RPE cells were treated with TGF-β1 at different
concentration(0 ng/ml, 0. 01 ng/ml, 0. 1 ng/ml, 1 ng/ml, 10 ng/ml) for 24 h, then
telomerase activity was detected by telomerase repeat amplification protocol (TRAP). The
expression of hTERT mRNA and TEP-1mRNA were detected by reverse transcription
polymerase chain reaction(RT-PCR).
Results: TRAP and RT-PCR showed when the concentration of TGF-β1 was gradually
increased, telomerase activity and the expression of hTERTmRNA were gradually reduced,
TEP1mRNA showed no apparent differential expression.
Conclusion: TGF-β1 can down-regulate telomerase activity and the expression of hTERT
mRNA, but no effection on TEP-1mRNA, hTERTmRNA expression was in accordance with
telomerase activity in hRPE cells, hTERT gene plays a crucial role in the expression of
telomerase activity, while TEP1 plays a much smaller role. Eye Science 2003; 19: 60- 64.
