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水稻尿卟啉原脱羧酶编码基因的鉴定和电子克隆 被引量：2

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摘要 尿卟啉原脱羧酶(UROD)在动植物体内广泛存在,编码该酶的基因在不同植物中都有很大的保守性.依据UROD基因序列的保守性,鉴定和克隆了水稻的UROD基因,并获得了该基因的全序列.该基因共有1041个核苷酸,可编码347个氨基酸.遗传分析表明,该基因位于水稻第3号染色体上,与其他植物进行同源性比较发现,基因的差异主要集中于基因的N末端,这一段序列具有引导尿卟啉原脱羧酶进入叶绿体的传输功能.进一步比较了不同植物间UROD基因的DNA和氨基酸序列变异,以探讨基因的衍变过程.
作者 徐吉臣 刘国振 李夏真 肖晗 李晓波 李宏昌 韦丽荣 朱立煌
机构地区 中国科学院遗传与发育生物学研究所 河北农业大学生命科学院
出处 《自然科学进展》 北大核心 2004年第1期64-69,共6页
基金 国家重点基础研究发展规划资助项目(批准号:G1999011604)
关键词 水稻 尿卟啉原脱羧酶 编码基因 基因鉴定 电子克隆 基因定位
分类号 S511 [农业科学—作物学] Q78 [生物学—分子生物学]
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参考文献10

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同被引文献37

  • 1范军,何晓梅,朱娟,朱苏文,程备久.对生玉米幼叶尿卟啉原脱羧酶的纯化及部分性质研究[J].激光生物学报,2006,15(2):148-153. 被引量：2
  • 2Badenas C., To-Figueras J., Phillips J.D., Warby C.A., Munoz C., and Herrero C., 2009, Identification and characterization of novel uroporphyrinogen decarboxylase gene mutations in a large series ofporphyria cutanea tarda patients and relatives, Clinical Genetics, 75:346-353.
  • 3Balmer Y., Koller A., de Val G., Manieri W., Schurmann P., and Buchanan B.B., 2003, Proteomics gives insight into the regulatory function of chloroplast thioredoxins, Proceedings of the National Academy of Sciences of the United States of America, 100(1): 370-375.
  • 4Berry-Lowe S.L., Grimm B., Smith M.A. and Kannangara C.G., 1992, Purification and characterization of glutamate 1-semialdehyde aminotransferase from barley expressed in Escherichia coli, Plant Physiology, 99(4): 1597-1603.
  • 5Fan J., Liu Q., Hao Q., Teng M.K., and Niu L.W., 2007, Crystal structure of uroporphyrinogen decarboxylase from Bacillus subtilis, Journal of Bacteriology, 189(9): 3573-3580.
  • 6Fan J., Wang D.Q., Liang Z., Guo M., Teng M.K., and Niu L.W., 2006, Maize uroporphyrinogen Ⅲ methyltransferase: overexpression of the functional gene fragments in Escherichia coli and one-step purification, Protein Expression and Purification, 46(1): 40-46.
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  • 9Hu G., Yalpani N., Briggs S.P., and Johal G.S., 1998, A porphyrin pathway impairment is responsible for the phenotype of a dominant disease lesion mimic mutant of maize, The Plant Cell, 10:1095-1105.
  • 10Ikegami A., Yoshimura N., Motohashi K., Takahashi S., Romano P.G., Hisabori T., Takamiya K., and Masuda T., 2007, The CHLI1 subunit ofA rabidopsis thaliona magnesium chelatase is a target protein of the chloroplast thioredoxin, Journal of Biological Chemistry, 282(27): 19282-19291.

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自然科学进展
2004年 第1期

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