生物检材中乌头碱的GC/MS分析

Detection of aconitine in biological samples by GC/MS

目的 建立GC/MS-SIM检测生物检材中乌头碱的定性方法。方法 以MSTFA作为衍生化试剂,吡啶为溶剂,于60℃反应30min,综合质谱图及保留时间定性分析。结果 经该方法测得乌头碱最小检出量为20ng(S/N≥100)。结论 GC/MS-SIM法适用于药材及生物检材中乌头碱的定性分析。

Objective To establish a method used for qualitative determination of aconitine in biological samples by GC/MS. Methods using MSTFA as derivative reagent and pyridine as solvent leaving in 60℃ for 30min then detected by GC/MS-SIM. Results The limit of detection of aconitine was 20ng (S/N≥100). Conclusion This method is sensitive, fast, and accurate. It is useful for forensic medical practice.