消退素D1对咪喹莫特诱导的小鼠银屑病模型的治疗作用

Therapeutic Effect of Resolvin D1 on Imiquimod-induced Mouse Psoriasis Model

目的探讨消退素D1对咪喹莫特诱导的小鼠银屑病模型的治疗作用。方法 30只小鼠分为模型组、实验组及对照组,模型组仅使用咪喹莫特乳膏外涂造模,实验组造模并腹腔内注射消退素D1,对照组造模并腹腔内注射生理盐水。肉眼及镜下观察各组小鼠皮肤组织的皮损严重程度,并采用RT-PCR检测各组小鼠皮肤组织内TNF-α、IL-17A、IL-23的mRNA表达情况。结果使用咪喹莫特造模后,小鼠背部、耳部皮肤可见鳞屑性红色斑块,上覆银白色鳞屑,有不同程度的浸润感。使用消退素D1处理的实验组小鼠皮肤上述情况明显改善,而生理盐水组皮损无明显改善。光学显微镜下观察,模型组小鼠皮肤可见表皮角化过度伴角化不全,棘层肥厚,表皮突向下延长,真皮乳头内可见毛细血管扩张伴炎症细胞浸润等银屑病特征性病理改变,实验组上述病理表现明显改善,对照组的病理表现则无明显改善。模型组皮肤组织内TNF-α、IL-17A、IL-23的mRNA表达水平明显升高,而实验组的表达水平较模型组明显下降,其差异有统计学意义(P<0.05),与对照组比较差异无统计学意义(P>0.05)。结论消退素D1对咪喹莫特诱导的小鼠银屑病模型具有一定的治疗作用,其作用机制可能是通过降低TNF-α、IL-17A、IL-23等炎症因子的水平。

Objective To explore the therapeutic effect of resolving D1 on imiquimod-induced mouse psoriasis model. Methods Thirty mice were divided into three groups: model group, experimental group and control group. In the model group, only imiquimod cream was used for external application, the experimental group was modeled and intraperitoneally injected with resolvin D1, and the control group was modeled and intraperitoneal injection with physiological saline. The severity of mouse skin lesions in each group was observed with the naked eye and microscopically, RT-PCR was used to detect the mRNA expression of TNF-α, IL-17 A, and IL-23 in the skin tissue of each group.Results After modeling with imiquimod, red and scaly patches were visible on the back and ears of the mice, covered with silvery white scales, with varying degrees of infiltration. The skin lesions of mice in the experimental group treated with resolvin D1 had obvious improvement, while no significant improvement in skin lesions was observed in the control saline group. Observed under the light microscope, hyperkeratosis of the epidermis with parakeratosis, acanthosis hypertrophy, epidermoid elongation down, and telangiectasia with inflammatory cell infiltration in dermis papillae and other characteristic pathological changes of psoriasis could be seen in the skin of the model group. The above-mentioned pathological manifestations of the experimental group were significantly improved, while the pathological manifestations of the control group were not significantly improved. The mRNA expression levels of TNF-α, IL-17 A, and IL-23 in the skin tissue of the model group increased significantly, while the expression levels in the experimental group decreased significantly compared with the model group(P<0.05). The difference between model group and control group was not statistically significant(P>0.05). Conclusion Resolvin D1 has a certain therapeutic effect on imiquimod-induced mouse psoriasis model, and its mechanism may be through the reduction of inflammatory factors such as TNF-α, IL-17 A, IL-23.