摘要
采用组织块分离法分离了短萼仪花病叶上的病原菌,通过针刺回接法确定了其致病性,进一步采用形态学和分子生物学相结合的方法对短萼仪花炭疽病的病原进行鉴定。同时,采用菌丝生长速率法测定了7种不同的杀菌剂对短萼仪花炭疽病病菌的室内毒力。结果表明,引起短萼仪花炭疽病的病原为胶孢炭疽菌(Colletotrichum gloeosporioide)。7种杀菌剂对短萼仪花炭疽病病菌的室内毒力明显不同。其中,97%吡唑醚菌酯表现出最好的抑制作用,EC_(50)为(9.67±1.20)μg/mL。
Pathogen on diseased leaves of Lysidice brevicalyx Wei was separated by tissue block separation method and confirmed by acupuncture method, which was further identified by morphological and molecular biology methods. Indoor toxicities of seven fungicides on pathogen of L. brevicalyx anthracnose were determined by mycelium growth rate method.The results showed that L. brevicalyx anthracnose was caused by Colletotrichum gloeosporioide, and different fungicides displayed different inhibitory effects on C. gloeosporioide. Among them, 97% pyraclostrobin showed best inhibition effect with EC50 value of 9.67±1.20 μg/mL.
作者
单体江
宋慧云
段志豪
王裕阳
王军
毛子翎
Shan Tijiang;Song Huiyun;Duan Zhihao;Wang Yuyang;Wang Jun;Mao Ziling(Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm/College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou Guangdong 510642,China;Guangdong Province Key Laboratory of Microbial Signals and Disease Control/South China Agricultural University,Guangzhou Guangdong 510642,China)
出处
《中国植保导刊》
北大核心
2019年第2期5-11,共7页
China Plant Protection
基金
广东省林业科技创新项目(2015KJCX043)
关键词
短萼仪花
分离鉴定
炭疽病病原菌
药剂筛选
Lysidice brevicalyx Wei
isolation and identification
anthracnose
fungicides screening
