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王锦蛇血清抗蛇毒有效成分的研究 被引量:1

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摘要 用硫酸铵分级,DEAE-萄萄糖凝胶A-50柱层析,CM-纤维素C-32柱层析及聚丙烯酰胺凝胶垂直板电泳等方法,自王锦蛇血清中分离纯化得到一个抗毒因子,经聚丙烯酰胺凝胶电泳鉴定,呈现一条区带。抗毒因子的分子量为67,000左右,等电点4.0,抗尖吻蝮蛇毒的致死活性提高7.5倍。
出处 《中草药》 CAS 1982年第4期5-7,共3页 Chinese Traditional and Herbal Drugs
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同被引文献10

  • 1曾仲意,张丽芬,冯健兰,曲敬来.犀角地黄汤化裁方对蛇伤患者血浆TT、PT、APTT的影响[J].蛇志,2005,17(2):76-78. 被引量:11
  • 2Omori-Satoh.T,Sadahiro.S, Ohsaka. A,et al. Purification and characterization of an antihemorrhagic factor in the serum of Trimeresurus flavoviridis a crotalid[J].Biochim. Biophys. Acta,1972,289: 414-426.
  • 3HUANG Jie-Tang, REN Xiao-Lan, ZHANG Mei, et al. Anti-ophidic toxin action of agkistrodon acutus serum[J].Acta Pharmacol Sin,1982,3(4): 245-250.
  • 4Lawan Chanhome, Orawan Khow, Tamotsu Omori-Satoh, et al. Antihemorrhagin in the blood serum of king cobra (Ophiophagus Hannah): purification and characterization [J]. Toxicon,2003,41: 1013-19.
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  • 6Yasuhiro Tomihara, Kazuo Yonaha, Masatoshi Noz-aki, et al. Purification of an antihemorrhagic factor from the serum of the non-venomous snake Dinodon semicarinatus [J]. Toxicon,1988,26:420-423.
  • 7Kondo.H, Kondo. S, Ikezawa. H,et al. Study on the quantitative method for determination of hemorrhagic activity of habu snake venom [J]. Jpn. J. Med. Sci.Biol,1960,13: 43-51.
  • 8Faria L de, Antunes E, et al. Pharmacological cha-racterization of the rat paw edema induced by Bothrops lanceolatus(Fer de lance) venom[J]. Toxicon, 2001, 39: 825-830.
  • 9黄接棠.毒蛇咬伤防治概述[M].合肥:安徽科学技术出版社(出版中),..
  • 10蛇血清抗五步蛇毒的实验研究[J].中草药,1979,10(3):35-36. 被引量:1

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