基于“药辅合一”的芍药甘草胃漂浮片对急性胃溃疡家兔的药效及机制研究

Pharmacodynamics and mechanism of Shaoyao Gancao Intragastric Floating Tablets based on unification of medicines and excipients

目的研究芍药甘草胃漂浮片"药辅合一"药效作用及其作用机制。方法家兔随机分为对照组、模型组、参梅养胃颗粒组(1.000 g/kg)、芍药甘草汤浸膏组(1.000 g/kg)、芍药甘草胃漂浮片低及高剂量组(1.000、1.500 g/kg)、含壳聚糖空白辅料组(1.000 g/kg)与不含壳聚糖空白辅料组(1.000 g/kg),每组6只,各组连续给药13 d后禁食24 h,除对照组外,各组家兔ig给予无水乙醇(2.5 mL/只)制备急性胃溃疡模型,造模1.5 h后取血液与胃组织,观察各组家兔胃组织病理损伤情况,检测各组家兔血清胃泌素(GAS)、丙二醛(MDA)、胃蛋白酶原(PGI)、表皮生长因子(EGF)及胃组织EGF、前列腺素E_2(PGE_2)、三叶因子1(TFF_1)水平。结果与模型组比较,各给药组均能改善急性胃溃疡家兔胃组织病理形态,家兔血清中EGF及胃组织中EGF、PGE_2、TFF_1水平均有不同程度升高,血清中GAS、MDA及PGⅠ水平均有不同程度降低。结论芍药甘草胃漂浮片中药物、辅料分用与合用均对无水乙醇型家兔胃溃疡具有一定治疗作用,作用机制可能与保护胃黏膜,改善保护因子、攻击因子之间失衡状态有关。

Objective To study the pharmacodynamic effect of 'unification of medicines and excipients' and the mechanism of action about Shaoyao Gancao Intragastric Floating Tablets(SGIFT). Methods Rabbits were randomly divided into control group and model group, Shenmei Yangwei Granule group(1.0 g/kg), Shaoyao Gancao Decoction extract group(1.0 g/kg), low-dose SGIFT group(1.0 g/kg), high-dose SGIFT group(1.5 g/kg), chitosan blank excipients group(1.0 g/kg). The acute gastric ulcer model was established in the blank excipient group without chitosan(1.0 g/kg). Six rats in each group, fasting for 24 h after 13 d of continuous administration, and gastric administration of anhydrous ethanol(2.5 mL/single). The blood and gastric tissue were taken out after 1.5 h to observe the pathological damage in each group. The expression of GAS, MDA, PGⅠ, epidermal growth factor(EGF) in serum and EGF, PGE2, TFF1 in gastric tissue of each group were detected. Results Compared with the model group, the other groups can improve the gastric histopathology of rabbits with acute gastric ulcer effectively. The content of EGF in serum and EGF, PGE2, and TFF1 in gastric tissue of rabbits was increased in varying degrees, and the content of GAS, MDA, and PGⅠ in serum was all decreased in different degrees. Conclusion SGIFT had certain therapeutic effects on rabbits with anhydrous ethanol gastric ulcer. The mechanism might be related to protecting gastric mucosa, increasing the content of protective factors, and reducing the content of attack factors.