连花清瘟胶囊的化学成分研究(Ⅲ)

Chemical components from Lianhua Qingwen Capsule(Ⅲ)

目的对连花清瘟胶囊化学成分进行系统研究,以期阐明该复方的物质基础。方法连花清瘟胶囊总浸膏经大孔树脂吸附,对30%乙醇洗脱部分采用中低压色谱和高压制备液相色谱对其进行分离和纯化,根据化合物的光谱数据对化学结构进行鉴定。结果连花清瘟胶囊总浸膏中分离得到18个化合物,分别鉴定为连翘酯苷A(1)、连翘酯苷I(2)、连翘酯苷H(3)、lugrandoside(4)、isolugrandoside(5)、ferruginoside A(6)、lianqiaoxinoside C(7)、calceolarioside C(8)、连翘酯苷E(9)、ferruginosideB(10)、D-苦杏仁苷(11)、L-苦杏仁苷(12)、sambunigrin(13)、cornoside(14)、4-hydroxy-4-methylenecarbomethoxy-cyclohexa-2,5-dienone(15)、鹅掌楸苷(16)、甘草素-7-O-β-D-葡萄糖苷(17)、3,4-二羟基苯甲醛(18)。结论化合物2～8、10、13～18均为首次从连花清瘟胶囊总浸膏中分离得到,化合物4～6、10、15、16均未见从复方单味药中分离的报道;首次采用二维核磁波谱技术,对化合物8在DMSO-d6溶剂中的核磁数据进行了归属;系统地阐述了连花清瘟胶囊总浸膏大极性部位的化学组成,进一步丰富了连花清瘟胶囊的化学信息。

Objective To study the chemical constituents of Lianhua Qingwen Capsule and illuminate its substance foundation.Methods The compounds were isolated and purified by LPLC and preparative HPLC from the 30%ethanol fraction of Lianhua Qingwen Capsule macroporous resin column chromatography.Their chemical structures were elucidated by the spectral analyses.Results 18 compounds were isolated and identified as forsythoside A(1),forsythoside I(2),forsythoside H(3),lugrandoside(4),isolugrandoside(5),ferruginoside A(6),lianqiaoxinoside C(7),calceolarioside C(8),forsythoside E(9),ferruginoside B(10),D-amygdaloside(11),L-amygdaloside(12),sambunigrin(13),cornoside(14),4-hydroxy-4-methylenecarbomethoxy-cyclohexa-2,5-dienone(15),liriodendrin(16),liquiritigenin-7-O-β-D-glucopyranoside(17),and 3,4-dihydroxy benzaldehyde(18).Conclusion Compounds 2—8,10,and 13—18 are isolated from Lianhua Qingwen Capsule for the first time,and compounds 4—6,10,15,and16 are isolated from single herb in Lianhua Qingwen Capsule compound for the first time.The spectral data in DMSO-d6 solution of compound 8 are reported firstly with 2 D NMR spectral data.The above results show the high polar chemical constitutions of Lianhua Qingwen Capsule,which provides more chemical information of Lianhua Qingwen Capsule.