蜣螂疏水壳聚糖的制备及其止血活性研究

Preparation of hydrophobic chitosan from Catharsius molossus and its hemostatic effect

目的制备蜣螂疏水壳聚糖,研究其止血性能。方法以蜣螂壳聚糖为原料,通过与月桂酸酐酰基化反应,进行疏水改性与表征,同时采用离体凝集实验与小鼠肝创面实验研究其凝血性能。结果制备的蜣螂疏水壳聚糖疏水基取代度为(11.69±0.14)%,高于疏水市售壳聚糖;离体凝集实验表明其与血液混合后质量分数达6.0 mg/g时即可快速凝血(3.42±0.39)s,较蜣螂壳聚糖与市售疏水壳聚糖效果更优;小鼠肝创面实验显示蜣螂疏水壳聚糖的止血时间较蜣螂壳聚糖与疏水市售壳聚糖明显缩短。结论采用疏水改性可明显提高壳聚糖的凝血性能,且蜣螂疏水壳聚糖最为优良,有望成为一种有巨大潜力的新型止血材料。

Objective To prepare hydrophobic chitosan from Catharsius molossus and study its hemostatic effect.Methods The chitosan from C.molossus was used as material to carry out hydrophobic modification and characterization by acylation with lauric anhydride.At the same time,the hemostatic effect was studied by in vitro agglutination test and mouse liver wound experiment.Results The prepared hydrophobic chitosan had a hydrophobic group substitution degree of(11.69±0.14)%,which was higher than that of commercially available hydrophobic chitosan.The in vitro agglutination experiment showed that it could coagulate in(3.42±0.39)s when mixed with blood at a concentration of 6.0 mg/g,which was better than chitosan and hydrophobically modified commercial chitosan.The mouse liver wound experiment showed that chitosan from C.molossus could shorten hemostatic time significantly compared with chitosan and hydrophobically modified commercial chitosan.Conclusion Hydrophobically modified chitosan can significantly improve the coagulation performance of chitosan,and the hydrophobic chitosan from C.molossus is excellent,and it is expected to be a new hemostatic material with great potential.