重楼根际及药用部位内生真菌多样性与群落结构差异分析

Fungal diversity and differential analysis of fungi community composition in Paris polyphylla var. chinensis rhizosphere and endophytic

目的寻找能促进宿主植物活性代谢产物合成的微生物,为后续功能菌群的建立及功能基因的发掘,提供理论支持。方法应用IlluminaMiseqPE250高通量测序技术对重楼根际土壤真菌、根及根茎内生真菌ITS1区进行序列测定,并进行生物信息学分析。结果测序结果共获得154228条序列,这些序列对应的根际土、根茎、根分别被聚类为408、119、57个OTUs。结论重楼根际及内生真菌多样性的关系为根际土>根茎>根,在重楼药用部位根茎中发现Mucoromycota门较非药用部位根中的门为特有门,Ascomycota门为优势门,Roseodiscus属真菌为重楼根茎内生真菌优势菌群,根茎和根部的特有种群及优势菌群可能与重楼活性产物合成密切相关。

Objective To find the microbes promoting the biosynthesis of active metabolites in host plant,which could provide theoretical support to the establishment of functional flora and discovery of key genes.Methods The ITS1 region of rhizosphere fungi and endophytic fungi from Paris polyphylla var.chinensis was sequenced by Illumina Miseq PE250 high-throughput sequencing technique,and the biological information was also analyzed.Results It was shown that the number of effective sequences for sample was 154 228,and these sequences were assigned to 408,119,and 57 OTUs with rhizosphere soil,rhizome,and root,respectively.Conclusion It was indicated that the diversity relationship between rhizosphere fungi and endophytic fungi of Paris polyphylla var.chinensis was rhizosphere>rhizome>root.It was found that there were more Mucoromycota Phylum in the rhizome than root,and the dominant population of endophytic fungi in rhizome of Paris polyphylla var.chinensis.belongs to Roseodiscus.Ascomycota is the dominant Phylum.It is suggested that the specific fungi may be an important microbial community affecting the biosynthesis of the active products in Paris polyphylla var.chinensis.