黄花败酱草的化学成分研究

Chemical constituents of Patrina scabiosaefolia

目的研究黄花败酱草Patrina scabiosaefolia的化学成分。方法采用硅胶、Sephadex LH-20、ODS柱色谱及制备型HPLC等色谱技术进行分离纯化,并根据理化数据和波谱学技术鉴定化合物的结构。结果从黄花败酱草的乙醇提取物中共分离得到18个化合物,其结构分别鉴定为6-甲氧基-7-(1′-二十一碳酰氧基-3′-羟基-2′-丙氧基)香豆素(1)、齐墩果酸(2)、熊果酸(3)、棕榈酸(4)、月桂酸(5)、α-生育醌(6)、山楂酸(7)、4-羟基-3-甲氧基苯甲醛(8)、2,6-二甲氧基苯醌(9)、3β-乙酰氧基-12-烯-28-熊果酸(10)、α-生育螺环B(11)、22E-3β-羟基-5α,6α-环氧麦角甾-22-烯-7-酮(12)、2α-羟基熊果酸(13)、亚油酸(14)、β-谷甾醇棕榈酸酯(15)、3-羟基-1-(4-羟基-3-甲氧基苯)-1-丙酮(16)、橄榄树脂素(17)、常春藤皂苷元(18)。结论化合物1为新化合物,命名为黄花败酱内酯。化合物6、8、9、11、12、16、17为首次从败酱属植物中分离得到。

Objective To study the chemical constituents of the herbs of Patrina scabiosaefolia.Methods Many column chromatographies such as silica gel,Sephadex LH-20,ODS column chromatography and preparative high-performance liquid chromatography were used to separate and purify the chemical constituents of P.scabiosaefolia.The structures of these compounds were identified by physical-chemical data and spectroscopic techniques.Results Eighteen compounds were isolated and purified from the ethanol extract of P.scabiosaefolia,and their structures were identified as follows:6-methoxy-7-(1′-henicosacyloxy-3′-hydroxy-2′-propoxy)coumarin(1),oleanolic acid(2),ursolic acid(3),palmitic acid(4),dodecanoic acid(5),α-tocopherolquinone(6),maslinic acid(7),4-hydroxy-3-methoxybenzaldehyde(8),2,6-dimethoxy benzoquinone(9),3β-acetyloxy-12-en-28-ursolic acid(10),α-tocospiro B(11),22 E-3β-hydroxy-5α,6α-epoxyergosta-22-en-7-one(12),2α-hydroxy ursolic acid(13),linoleic acid(14),β-sitosterol palmitate(15),3-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-1-propanone(16),burselignan(17),and hederagenin(18).Conclusion Compound 1 is a new compound named scabiosaelactone and compounds 6,8,9,11,12,16,and 17 are isolated from the genus Patrina for the first time.