野生白及资源遗传多样性的SRAP分析

Genetic diversity of wild Bletilla striata based on SRAP markers

目的对不同来源的野生白及遗传多样性进行分析,为白及种质的选育、保护和开发提供参考。方法以全国不同地区的50份野生白及样品为材料,利用序列相关多态性扩增(SRAP)分子标记技术进行遗传多样性分析,使用UPGMA法进行白及亲缘关系的聚类分析。结果利用筛选出的15对SRAP引物组合,50份样品扩增获得共117个条带,平均每个引物产生7.8个条带,其中多态性条带106条,多态性比率为90.60%。聚类结果表明供试50份白及样品的遗传相似系数为0.59～0.87,以遗传系数0.66为基准可以分成6大类。遗传距离最近的2份紫花白及分别来自云南丽江市2号和湖北京山县2号,其遗传距离与地理距离无直接关联。结论野生白及资源间存在较丰富的遗传多样性,且地理距离与遗传距离并无直接关联,SRAP技术可为白及的资源保护、品种选育提供理论依据,为白及后续开发利用提供参考。

Objective The genetic diversity of wild Bletilla striata from different sources was analyzed in order to provide the reference value for breeding,protection and development of B.striata germplasm in the future.Methods Genetic diversity of 50 wild B.striatasamples was identified by SRAP molecular marker technique,and the genetic relationship was analyzed by UPGMA method.Results A total of 117 bands were amplified from 50 samples by using 15 SRAP primer combinations,with an average of 7.8 bands per primer.Among them,106 bands were polymorphic,with a polymorphism rate of 90.60%.The clustering results showed that the genetic similarity coefficient of 50 B.striata samples was 0.59—0.87,which could be divided into six categories based on the genetic coefficient of 0.66.The genetic distance of two pieces of B.striata from No.2 Lijiang,Yunnan and No.2 Jingshan County,Hubei was not directly related to geographical distance.Conclusion There are abundant genetic diversity among wild B.striata resources,and there is no direct relationship between geographical distance and genetic distance.SRAP technology can provide theoretical basis for the protection of B.striata resources and variety breeding,which provides reference for the subsequent development and utilization of B.striata.