一测多评法同时测定新疆软紫草中6种萘醌类成分

Simultaneous determination of six anthraquinone components in Arnebia euchroma by QAMS

目的建立新疆软紫草Arnebia euchroma中紫草素、乙酰紫草素、去氧紫草素、异丁酰紫草素、β-乙酰氧基异戊酰紫草素和β,β′-二甲基丙烯酰紫草素6种萘醌类成分的一测多评测定方法。方法采用高分辨LC-MS对软紫草药材中的主要萘醌类色谱峰进行鉴定。采用HPLC法,以乙酰紫草素为内参物,建立了乙酰紫草素与紫草素、去氧紫草素、异丁酰紫草素、β-乙酰氧基异戊酰紫草素和β,β′-二甲基丙烯酰紫草素的相对校正因子,并考察了相对校正因子的耐用性和重现性。比较了外标法和一测多评法对新疆软紫草中6种成分的测定结果。结果各相对校正因子重复性良好,一测多评法测定结果与外标法测定结果无显著差异。结论建立同时测定6种成分的一测多评法控制新疆软紫草药材的质量是准确、可靠的。

Objective To establish a new method for the quantitative analysis of multi-components by single-marker(QAMS)to simultaneous determine six naphthoquinone components in Arnebia euchroma.Methods The chromatographic peaks of the main naphthoquinone components in A.euchroma were identified by high resolution LC-MS.The acetyl Shikonin was used as internal marker to calculate the relative correlation factors(RCF)of deoxyshikonin,isobutyrylshikonin,β-acetoxyisovalerate shikonin,andβ,β′-dimethylacryloyl shikonin by HPLC,and examine the durability and reproducibility of the RCF.The external standard method and QAMS were compared to determine the six components in A.euchroma.Results The repeatability of RCF was good.The results calculated with QAMS were consistent with the results by the external standard method.Conclusion The QAMS method for simultaneously measuring the content of six components is accurate and reliable to evaluate the quality of A.euchroma.