忍冬不同部位UPLC指纹图谱及化学模式识别研究

Wavelength switching of UPLC fingerprint and chemical pattern recognition of different medical parts of Lonicera japonica

目的 采用UPLC法建立金银花、忍冬叶和忍冬藤指纹图谱,对忍冬不同部位采用指纹图谱相似度评价及聚类分析和主成分分析等化学模式识别技术进行研究,以期为忍冬药材的综合利用提供科学依据。方法 采用ACQUITYUPLC BEH C18(100 mm×2.1 mm,1.7μm)色谱柱,以乙腈-0.1%磷酸溶液为流动相梯度洗脱,体积流量0.3 mL/min,柱温30℃,样品室温度8℃,检测波长326、238、350 nm,进样量1μL。结果 28批样品有14个共有峰,与对照品色谱峰比对,指认了10个色谱峰,分别为新绿原酸、绿原酸、隐绿原酸、咖啡酸、马钱苷、芦丁、木樨草苷、异绿原酸B、异绿原酸A和异绿原酸C,金银花(10批)、忍冬叶(9批)和忍冬藤(9批)化学成分组成及量均存在一定差异。采用聚类分析及主成分分析从化学成分上揭示了忍冬不同部位28批样品的相似性及差异性。结论 金银花与忍冬叶化学成分较为相似,而与忍冬藤间存在差异,所建立的指纹图谱方法可为金银花、忍冬叶、忍冬藤的质量控制提供参考。

Objective To study Lonicerae Japonicae Flos,Lonicerae Japonicae Caulis,and Lonicerae Japonicae Leaves by UPLC method,and study the different parts of Lonicera japonica by the fingerprint similarity evaluation,cluster analysis,principal component analysis,and other chemical pattern recognition technologies,in order to provide scientific basis for the comprehensive utilization of L.japonica.Methods The method was carried out on an ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7μm)by a gradient elution using acetonitrile and 0.1%phosphoric acid.The flow rate was 0.3 mL/min,The column temperature was 30℃.The sample room temperature was 8℃.The detection wavelengths were 326 nm,238 nm and 250 nm,and the injection volume was 1μL.Results The UPLC fingerprint of 28 batches of samples from different parts of Lonicerae Japonicae were set up and 14 common peaks were obtained.They were new chlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,loganin,rutinum,luteoloside,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C.There were some differences in chemical composition and quantity of Lonicerae Japonicae Flos,Lonicerae Japonicae leaves,and Lonicerae Japonicae Caulis.PCA and cluster analysis revealed the similarity and difference of 28 batches of samples from different parts of L.japonica.Conclusion The combination of clustering analysis and principle component analysis could be used to confirm that the chemical constituents of Lonicerae Japonicae Flos and Lonicerae Japonicae leaves were similar,but there was a difference between Lonicerae Japonicae Flos and Lonicerae Japonicae Caulis.The established fingerprint method can provide a reference for the quality control of Lonicerae Japonicae Flos,Lonicerae Japonicae leaves,and Lonicerae Japonicae Caulis.