亚硒酸钠对人胃腺癌细胞线粒体结构与功能的影响

Effect of Sodium Selenite on Mitochondrial Structure and Function of Human Gastric Adenocarcinoma Cells

3×10^(-4)mol/l亚硒酸钠处理人胃腺癌MGc80-3细胞后,线粒体结构发生了明显变化:线粒体结构典型,多呈短棒状,大小较一致,分布均匀,线粒体嵴明显增多,密度和长度增大,排列方向较一致,线粒体空泡化和嵴扩张现象显著减少,表现出与人正常胃粘膜原代培养细胞线粒体基本相似的特征,进一步分析与线粒体相关的细胞色素氧化酶活性,发现亚硒酸钠可以显著提高该酶活性,由此表明,亚硒酸钠通过改变胃癌细胞线粒体的恶性结构,使线粒体功能恢复正常,调节了细胞代谢机能,从而诱导胃癌细胞向正常细胞方向分化。

After treating gastric carcinoma cells (MGc80-3) with 3×10-6 mol/l sodium selenite, there were many changes:mitochondrial structures were typical and in short-rod-like form,mitochondria distributed equally,and their swelling disappeared,mitochondrial cristae increased and arranged regularly, crista length enlarged and crista expansion decreased obviously. These features were similar to those of their corresponding normal cell's mitochondria,Further analysis showed that sodium selenite could increase cytochrome oxidase' s activity remarkably. These results confirmed that sodium selenite coulst change mitochondrial function through reversing mitochondrial malignant structure in gastric carcinoma cell,It could regulate cell's metabolism and induce cancer cells differentiated towards the normal cells.